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Literature summary for 4.2.1.20 extracted from
- An engineered tryptophan synthase opens new enzymatic pathways to beta-methyltryptophan and derivatives (2017), ChemBioChem, 18, 382-386 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the reaction of mutant L166V with L-amino acid oxidase, halogenase enzymes and palladium chemocatalysts provides access to further D-configured and regioselectively halogenated or arylated beta-methyltryptophan derivatives | Salmonella enterica subsp. enterica serovar Typhimurium |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| L166A | mutation in beta-subunit, 3fold increase in catalytic efficiency with L-threonine as substrate, decrease in efficiency with L-serine | Salmonella enterica subsp. enterica serovar Typhimurium |
| L166V | mutation in beta-subunit, 7fold increase in catalytic efficiency with L-threonine as substrate, decrease in efficiency with L-serine | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
L-serine | mutant L166V, pH not specified in the publication, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium |  |
| 1.6 | - |
L-serine | wild-type, pH not specified in the publication, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 52 | - |
L-serine | mutant L166A, pH not specified in the publication, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 210 | - |
L-threonine | wild-type, pH not specified in the publication, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 310 | - |
L-threonine | mutant L166V, pH not specified in the publication, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 340 | - |
L-threonine | mutant L166A, pH not specified in the publication, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-serine + indole | - |
Salmonella enterica subsp. enterica serovar Typhimurium | L-tryptophan + H2O | - |
? | |
| L-serine + indole | - |
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 37845 | L-tryptophan + H2O | - |
? | |
| L-threonine + 2-methylindole | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 2-methyl-(2S,3S)-beta-methyltryptophan + H2O | - |
? | |
| L-threonine + 2-methylindole | - |
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 37845 | 2-methyl-(2S,3S)-beta-methyltryptophan + H2O | - |
? | |
| L-threonine + 4-fluoroindole | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 4-fluoro-(2S,3S)-beta-methyltryptophan + H2O | - |
? | |
| L-threonine + 4-fluoroindole | - |
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 37845 | 4-fluoro-(2S,3S)-beta-methyltryptophan + H2O | - |
? | |
| L-threonine + 4-methylindole | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 4-methyl-(2S,3S)-beta-methyltryptophan + H2O | - |
? | |
| L-threonine + 4-methylindole | - |
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 37845 | 4-methyl-(2S,3S)-beta-methyltryptophan + H2O | - |
? | |
| L-threonine + 6-methylindole | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 6-methyl-(2S,3S)-beta-methyltryptophan + H2O | - |
? | |
| L-threonine + 7-chloroindole | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 7-chloro-(2S,3S)-beta-methyltryptophan + H2O | - |
? | |
| L-threonine + 7-fluoroindole | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 7-fluoro-(2S,3S)-beta-methyltryptophan + H2O | - |
? | |
| L-threonine + 7-methoxyindole | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 7-methoxy-(2S,3S)-beta-methyltryptophan + H2O | - |
? | |
| L-threonine + 7-methylindole | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 7-methyl-(2S,3S)-beta-methyltryptophan + H2O | - |
? | |
| L-threonine + indole | - |
Salmonella enterica subsp. enterica serovar Typhimurium | (2S,3S)-beta-methyltryptophan + H2O | - |
? | |
| L-threonine + indole | - |
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 37845 | (2S,3S)-beta-methyltryptophan + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TRPS | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0007 | - |
L-threonine | wild-type, pH not specified in the publication, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.0035 | - |
L-threonine | mutant L166A, pH not specified in the publication, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.007 | - |
L-threonine | mutant L166V, pH not specified in the publication, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.0817 | - |
L-serine | mutant L166V, pH not specified in the publication, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.183 | - |
L-serine | mutant L166A, pH not specified in the publication, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.35 | - |
L-serine | wild-type, pH not specified in the publication, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000032 | - |
L-threonine | wild-type, pH not specified in the publication, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.0000102 | - |
L-threonine | mutant L166A, pH not specified in the publication, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.000023 | - |
L-threonine | mutant L166V, pH not specified in the publication, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.0037 | - |
L-serine | mutant L166A, pH not specified in the publication, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.06 | - |
L-serine | mutant L166V, pH not specified in the publication, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.217 | - |
L-serine | wild-type, pH not specified in the publication, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
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