Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli T7-express cells | Salmonella enterica subsp. enterica serovar Typhimurium |
Protein Variants | Comment | Organism |
---|---|---|
D13N/F22C/T24A/K35E/D46N/D56N/P62S/E90K/G98C/L177D/Q210L/A222T | the mutant shows phosphoribosylanthranilate isomerase activity | Salmonella enterica subsp. enterica serovar Typhimurium |
D13N/F22C/T24A/K35E/D56N/P62S/E90K/G98C/L144I/L177D/Q210L/I240V | the mutant shows phosphoribosylanthranilate isomerase activity | Salmonella enterica subsp. enterica serovar Typhimurium |
D13N/F22C/T24A/K35E/D56N/P62S/G98C/L177D/Q210L | the mutant shows phosphoribosylanthranilate isomerase activity | Salmonella enterica subsp. enterica serovar Typhimurium |
D13N/F22C/T24A/T39A/D60N/G61S/P62S/N66S/E90K/G98C/L177D/Q210L/A222T/I240V | the mutant shows phosphoribosylanthranilate isomerase activity | Salmonella enterica subsp. enterica serovar Typhimurium |
F22C/T24A/K35E/D56N/P62S/E90K/G98C | the mutant shows phosphoribosylanthranilate isomerase activity | Salmonella enterica subsp. enterica serovar Typhimurium |
F22C/T24A/K35E/D56N/P62S/E90K/L144I/L177D/I240V | the mutant shows phosphoribosylanthranilate isomerase activity | Salmonella enterica subsp. enterica serovar Typhimurium |
F22C/T24A/K35E/T39A/D56N/G61S/E90K/G98C/L177D/Q210L/I240V | the mutant shows phosphoribosylanthranilate isomerase activity | Salmonella enterica subsp. enterica serovar Typhimurium |
F22Y/T39A/G61S/G98C/D161V/Y203N/A222T | the mutant shows phosphoribosylanthranilate isomerase activity | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | - |
L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | P00929 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-chelate affinity column chromatography | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | - |
r |
Synonyms | Comment | Organism |
---|---|---|
TrpA | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
tryptophan synthase alpha-subunit | - |
Salmonella enterica subsp. enterica serovar Typhimurium |