Cloned (Comment) | Organism |
---|---|
over-expressed in Mycobacterium tuberculosis H37Rv as a His-tagged fusion protein | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
H100A | Km (indole), (L-serine) higher than wild-type, kcat (indole), (L-serine) significantly decreased compared to wild-type | Mycobacterium tuberculosis |
N250A | Km (indole), (L-serine) higher than wild-type, kcat (indole), (L-serine) lower compared to wild-type | Mycobacterium tuberculosis |
Q128A | Km (indole), (L-serine) higher than wild-type, kcat (indole) slightly lower than wild-type, kcat (L-serine) lower compared to wild-type | Mycobacterium tuberculosis |
Q364A | Km (indole), (L-serine) higher than wild-type, kcat (indole), (L-serine) significantly decreased compared to wild-type | Mycobacterium tuberculosis |
S249A | Km (indole), (L-serine) higher than wild-type, kcat (indole), (L-serine) lower compared to wild-type | Mycobacterium tuberculosis |
S390A | Km (indole), (L-serine) higher than wild-type, kcat (indole), (L-serine) lower compared to wild-type | Mycobacterium tuberculosis |
S99A | Km (indole), (L-serine) higher than wild-type, kcat (indole), (L-serine) lower compared to wild-type | Mycobacterium tuberculosis |
T204A | Km (indole), (L-serine) higher than wild-type, kcat (indole), (L-serine) lower compared to wild-type | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.178 | - |
L-serine | - |
Mycobacterium tuberculosis | |
0.288 | - |
indole | - |
Mycobacterium tuberculosis | |
0.983 | - |
L-serine | mutant T204A | Mycobacterium tuberculosis | |
0.997 | - |
indole | mutant Q128A | Mycobacterium tuberculosis | |
1.013 | - |
indole | mutant T204A | Mycobacterium tuberculosis | |
1.052 | - |
L-serine | mutant Q128A | Mycobacterium tuberculosis | |
1.235 | - |
L-serine | mutant S249A | Mycobacterium tuberculosis | |
1.485 | - |
indole | mutant S249A | Mycobacterium tuberculosis | |
1.683 | - |
L-serine | mutant S99A | Mycobacterium tuberculosis | |
1.85 | - |
L-serine | mutant N250A | Mycobacterium tuberculosis | |
1.911 | - |
L-serine | mutant S390A | Mycobacterium tuberculosis | |
2.083 | - |
indole | mutant S390A | Mycobacterium tuberculosis | |
2.342 | - |
indole | mutant S99A | Mycobacterium tuberculosis | |
2.86 | - |
indole | mutant N250A | Mycobacterium tuberculosis | |
2.906 | - |
indole | mutant Q364A | Mycobacterium tuberculosis | |
4 | - |
L-serine | mutant Q364A | Mycobacterium tuberculosis | |
4.471 | - |
indole | mutant H100A | Mycobacterium tuberculosis | |
15.8 | - |
L-serine | mutant H100A | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | highest activity with 0.18 M Mg2+ | Mycobacterium tuberculosis | |
Na+ | highest activity with 0.15 M Na+ | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
SDS-PAGE, recombinant protein | Mycobacterium tuberculosis |
50220 | - |
mass spectroscopy | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using metal affinity chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
indole + L-serine | - |
Mycobacterium tuberculosis | L-tryptophan + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
beta subunit of tryptophan synthase | - |
Mycobacterium tuberculosis |
Rv1612 | - |
Mycobacterium tuberculosis |
TrB | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | the enzyme activity decreases significantly when the temperature is above 40°C. The activity of enzyme incubated at 80°C is below 10% of that incubated at 10°C | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.987 | - |
L-serine | mutant H100A | Mycobacterium tuberculosis | |
2.531 | - |
indole | mutant H100A | Mycobacterium tuberculosis | |
3.462 | - |
L-serine | mutant Q364A | Mycobacterium tuberculosis | |
6.427 | - |
indole | mutant Q364A | Mycobacterium tuberculosis | |
7.106 | - |
L-serine | mutant N250A | Mycobacterium tuberculosis | |
10.69 | - |
L-serine | mutant S249A | Mycobacterium tuberculosis | |
13.22 | - |
L-serine | mutant S99A | Mycobacterium tuberculosis | |
16.35 | - |
L-serine | mutant T204A | Mycobacterium tuberculosis | |
20.48 | - |
L-serine | mutant S390A | Mycobacterium tuberculosis | |
21.14 | - |
indole | mutant S99A | Mycobacterium tuberculosis | |
21.47 | - |
L-serine | mutant Q128A | Mycobacterium tuberculosis | |
26.84 | - |
indole | mutant S249A | Mycobacterium tuberculosis | |
34.73 | - |
indole | mutant S390A | Mycobacterium tuberculosis | |
40.18 | - |
indole | mutant N250A | Mycobacterium tuberculosis | |
48.21 | - |
L-serine | - |
Mycobacterium tuberculosis | |
50.65 | - |
indole | mutant T204A | Mycobacterium tuberculosis | |
68.43 | - |
indole | mutant Q128A | Mycobacterium tuberculosis | |
70.29 | - |
indole | - |
Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
physiological function | structure analysis indicate that Mycobacterium tuberculosis TrpB exhibits a typical beta/alpha barrel structure | Mycobacterium tuberculosis |