Literature summary for 4.2.1.20 extracted from

Ngo, H.; Harris, R.; Kimmich, N.; Casino, P.; Niks, D.; Blumenstein, L.; Barends, T.R.; Kulik, V.; Weyand, M.; Schlichting, I.; Dunn, M.F.
Synthesis and characterization of allosteric probes of substrate channeling in the tryptophan synthase bienzyme complex (2007), Biochemistry, 46, 7713-7727.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
soaking native crystals for 10 min in a solution containing 90 mM Bis-Tris-propane (pH 7.8), 150 mM NaCl, 15% (w/v) PEG 8000, and 20% glycerol	Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism	UniProt	Comment	Textmining
Salmonella enterica subsp. enterica serovar Typhimurium	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-serine + indole	-	Salmonella enterica subsp. enterica serovar Typhimurium	L-tryptophan + H2O	-	?

Subunits

Subunits	Comment	Organism
tetramer	X-ray crystallography, consists of an alpha2beta2 bienzyme complex with alphabeta dimeric units assembled as the tetrameric species via the beta-beta subunit interface, each alpha-subunit catalyzes the cleavage of 3-indole-D-glycerol 3'-phosphate to indole and D-glyceraldehyde 3-phosphate, the pyridoxal phosphate requiring beta-subunit catalyzes a beta-replacement reaction in which indole replaces the hydroxyl of L-Ser, giving L-Trp	Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

Synonyms	Comment	Organism
alpha2beta2 tryptophan synthase	consists of an alpha2beta2 bienzyme complex with alphabeta dimeric units assembled as the tetrameric species via the beta-beta subunit interface, each alpha-subunit catalyzes the cleavage of 3-indole-D-glycerol 3'-phosphate to indole and D-glyceraldehyde 3-phosphate, the pyridoxal phosphate requiring beta-subunit catalyzes a beta-replacement reaction in which indole replaces the hydroxyl of L-Ser, giving L-Trp	Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Salmonella enterica subsp. enterica serovar Typhimurium

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Release 2023.1 (January 2023)