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Literature summary for 4.2.1.20 extracted from

  • Raboni, S.; Mozzarelli, A.; Cook, P.F.
    Control of ionizable residues in the catalytic mechanism of tryptophan synthase from Salmonella typhimurium (2007), Biochemistry, 46, 13223-13234.
    View publication on PubMed
Search for more literature for 4.2.1.20

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 L-serine in the presence of 250 mM NaCl, at pH 8.5 and 25°C Salmonella enterica subsp. enterica serovar Typhimurium
0.27
-
 L-serine in the presence of 100 mM KCl, at pH 8.5 and 25°C Salmonella enterica subsp. enterica serovar Typhimurium
0.52
-
 L-serine in the presence of 250 mM NaCl, at pH 7.8 and 25°C Salmonella enterica subsp. enterica serovar Typhimurium
0.58
-
 L-serine in the presence of 100 mM KCl, at pH 7.8 and 25°C Salmonella enterica subsp. enterica serovar Typhimurium
1.6
-
 L-serine without monovalent cations, at pH 6.5 and 25°C Salmonella enterica subsp. enterica serovar Typhimurium
1.6
-
 L-serine without monovalent cations, at pH 7.8 and 25°C Salmonella enterica subsp. enterica serovar Typhimurium
1.89
-
 L-serine in the presence of 100 mM KCl, at pH 6.5 and 25°C Salmonella enterica subsp. enterica serovar Typhimurium
1.9
-
 L-serine in the presence of 100 mM CsCl, at pH 8.5 and 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7
-
 L-serine without monovalent cations, at pH 8.5 and 25°C Salmonella enterica subsp. enterica serovar Typhimurium
4.78
-
 L-serine in the presence of 250 mM NaCl, at pH 6.5 and 25°C Salmonella enterica subsp. enterica serovar Typhimurium
5.6
-
 L-serine in the presence of 100 mM CsCl, at pH 7.8 and 25°C Salmonella enterica subsp. enterica serovar Typhimurium
9
-
 L-serine in the presence of 100 mM CsCl, at pH 6.5 and 25°C Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions Comment Organism Structure
Cs+ stimulates activity at 100 mM Salmonella enterica subsp. enterica serovar Typhimurium
K+ stimulates activity at 100 mM Salmonella enterica subsp. enterica serovar Typhimurium
Na+ stimulates activity at 250 mM Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + indole
-
 Salmonella enterica subsp. enterica serovar Typhimurium L-tryptophan + H2O
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 L-serine in the presence of 100 mM CsCl, at pH 8.5 and 25°C Salmonella enterica subsp. enterica serovar Typhimurium
0.24
-
 L-serine without monovalent cations, at pH 6.5 and 25°C Salmonella enterica subsp. enterica serovar Typhimurium
0.91
-
 L-serine without monovalent cations, at pH 7.8 and 25°C Salmonella enterica subsp. enterica serovar Typhimurium
1.37
-
 L-serine without monovalent cations, at pH 8.5 and 25°C Salmonella enterica subsp. enterica serovar Typhimurium
1.9
-
 L-serine in the presence of 250 mM NaCl, at pH 6.5 and 25°C Salmonella enterica subsp. enterica serovar Typhimurium
1.9
-
 L-serine in the presence of 250 mM NaCl, at pH 8.5 and 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.08
-
 L-serine in the presence of 100 mM KCl, at pH 6.5 and 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.8
-
 L-serine in the presence of 250 mM NaCl, at pH 7.8 and 25°C Salmonella enterica subsp. enterica serovar Typhimurium
4
-
 L-serine in the presence of 100 mM KCl, at pH 8.5 and 25°C Salmonella enterica subsp. enterica serovar Typhimurium
4.8
-
 L-serine in the presence of 100 mM KCl, at pH 7.8 and 25°C Salmonella enterica subsp. enterica serovar Typhimurium
6
-
 L-serine in the presence of 100 mM CsCl, at pH 6.5 and 25°C Salmonella enterica subsp. enterica serovar Typhimurium
6.15
-
 L-serine in the presence of 100 mM CsCl, at pH 8.5 and 25°C Salmonella enterica subsp. enterica serovar Typhimurium
8.7
-
 L-serine in the presence of 100 mM CsCl, at pH 7.8 and 25°C Salmonella enterica subsp. enterica serovar Typhimurium

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium