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Literature summary for 4.2.1.20 extracted from

  • Fan, Y.X.; McPhie, P.; Miles, E.W.
    Thermal repair of tryptophan synthase mutations in a regulatory intersubunit salt bridge. Evidence from Arrhenius plots, absorption spectra, and primary kinetic isotope effects (2000), J. Biol. Chem., 275, 20302-20307.
    View publication on PubMed
Search for more literature for 4.2.1.20

Protein Variants

Protein Variants Comment Organism
D56A site-directed mutagenesis, mutation in the alpha-subunit, replacement of the residue, which builds a salt bridge to the alpha-subunit, has deleterious effects, which can be repaired by increased temperature in combination with CsCl or NaCl plus alpha-glycerol 3-phosphate, an alpha-ligand, alteration of temperature dependence of the enzyme complex Salmonella enterica subsp. enterica serovar Typhimurium
K167T site-directed mutagenesis, mutation in the beta-subunit, replacement of the residue, which builds a salt bridge to the alpha-subunit, has deleterious effects, which can be repaired by increased temperature in combination with CsCl or NaCl plus alpha-glycerol 3-phosphate, an alpha-ligand, alteration of temperature dependence of the enzyme complex Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors Comment Organism Structure
DL-glycerol 3-phosphate wild-type and mutant enzymes, in presence of monovalent cations Salmonella enterica subsp. enterica serovar Typhimurium
Na+ mutants D56A and K167T Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions Comment Organism Structure
Cs+ activation of mutants D56A and K167T Salmonella enterica subsp. enterica serovar Typhimurium
additional information monovalent cations are involved in the thermal stability and activity equilibrium of the enzyme complex, overview Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Reaction

Reaction Comment Organism Reaction ID
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8), reaction mechanism, formation of intermediates in the alpha-reaction Salmonella enterica subsp. enterica serovar Typhimurium
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
70
-
 mutant K167T, in presence of Na+ Salmonella enterica subsp. enterica serovar Typhimurium
75
-
 mutant D56A, in presence of Na+ Salmonella enterica subsp. enterica serovar Typhimurium
1310
-
 wild-type enzyme, in presence of Na+ Salmonella enterica subsp. enterica serovar Typhimurium
1400
-
 wild-type enzyme, in presence of Cs+ Salmonella enterica subsp. enterica serovar Typhimurium
1650
-
 mutant K167T, in presence of Cs+ Salmonella enterica subsp. enterica serovar Typhimurium
2200
-
 mutant D56A, in presence of Cs+ Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(indol-3-yl)glycerol 3-phosphate alpha-subunit of the bienzyme complex, alpha-reaction Salmonella enterica subsp. enterica serovar Typhimurium D-glyceraldehyde 3-phosphate + indole
-
 ?
L-serine + indole beta-subunit of the bienzyme complex, beta-reaction Salmonella enterica subsp. enterica serovar Typhimurium L-tryptophan + H2O
-
 ?

Subunits

Subunits Comment Organism
More structure and conformation regulating the activity and allosteric communication in the enzyme complex, modeling of the high activity closed form and the low activity open form Salmonella enterica subsp. enterica serovar Typhimurium
tetramer alpha2beta2 complex Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
 temperature-dependent changes in equilibrium distribution of enzyme-substrate intermediates and in primary kinetic isotope effect, D56 and K167 are involved Salmonella enterica subsp. enterica serovar Typhimurium