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Literature summary for 4.2.1.20 extracted from
- Molecular dissection of the folding mechanism of the alpha subunit of tryptophan synthase: an amino-terminal autonomous folding unit controls several rate-limiting steps in the folding of a single domain protein (1999), Biochemistry, 38, 10205-10214.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination of the alpha-subunit of the enzyme, overexpression of the alpha-subunit and the larger N-terminal part of the alpha-subunit, amino acid residues 1-188, in inclusion bodies | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant alpha-subunit and N-terminal part of the alpha-subunit of the enzyme, to over 95% purity | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8) | Escherichia coli |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| cooperative unfolding and 2-phase refolding mechanism and kinetics of the alpha-subunit and the N-terminas of the alpha-subunit alone, denaturing by urea at 25°C | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-(indol-3-yl)glycerol 3-phosphate | alpha-subunit of the bienzyme complex, alpha-reaction | Escherichia coli | D-glyceraldehyde 3-phosphate + indole | - |
? |  |
| L-serine + indole | beta-subunit of the bienzyme complex, beta-reaction | Escherichia coli | L-tryptophan + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | molecular dissection of the alpha-subunit | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alphaTS | - |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermodynamic stability of alpha-subunit and N-terminal part of the alpha-subunit during folding and unfolding | Escherichia coli |
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Release 2023.1 (January 2023)
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