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Literature summary for 4.2.1.2 extracted from

  • Zubimendi, J.P.; Martinatto, A.; Valacco, M.P.; Moreno, S.; Andreo, C.S.; Drincovich, M.F.; Tronconi, M.A.
    The complex allosteric and redox regulation of the fumarate hydratase and malate dehydratase reactions of Arabidopsis thaliana fumarase 1 and 2 gives clues for understanding the massive accumulation of fumarate (2018), FEBS J., 285, 2205-2224 .
    View publication on PubMed
Search for more literature for 4.2.1.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 fumarate pH 7.7, 30°C Arabidopsis thaliana
0.5
-
 fumarate pH 7.7, 30°C Arabidopsis thaliana
0.5
-
 fumarate presence of 3 mM oxalacetate, pH 8.0, 30°C Arabidopsis thaliana
0.5
-
 L-malate presence of 3 mM oxalacetate, pH 8.0, 30°C Arabidopsis thaliana
0.5
-
 fumarate presence of 3 mM oxaloacetate, pH 8.0, 30°C Arabidopsis thaliana
0.6
-
 L-malate pH 8.2, 30°C Arabidopsis thaliana
0.6
-
 L-malate presence of 3 mM oxaloacetate, pH 8.0, 30°C Arabidopsis thaliana
0.7
-
 fumarate pH 8.0, 30°C Arabidopsis thaliana
1.8
-
 L-malate pH 8.0, 30°C Arabidopsis thaliana
1.9
-
 L-malate pH 8.2, 30°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Arabidopsis thaliana 5829
-
mitochondrion
-
 Arabidopsis thaliana 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
195000
-
 gel filtration Arabidopsis thaliana
216000
-
 gel filtration Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P93033
-
-
Arabidopsis thaliana Q9FI53
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fumarate + H2O
-
 Arabidopsis thaliana L-malate
-
 r
L-malate
-
 Arabidopsis thaliana fumarate + H2O
-
 r
additional information fumarate hydratase activity is favored over the malate dehydratase activity. Presence of oxalacetate, glutamine, and/or asparagine cause the malate dehydratase reaction to become preferred over the fumarate hydratase reaction Arabidopsis thaliana ?
-
 ?
additional information fumarate hydratase activity is favored over the malate dehydratase activity. Presence of oxaloacetate, glutamine, and/or asparagine cause the malate dehydratase reaction to become preferred over the fumarate hydratase reaction Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 58000, SDS-PAGE Arabidopsis thaliana
tetramer 4 * 54000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
At2g47510
-
 Arabidopsis thaliana
At5g50950
-
 Arabidopsis thaliana
FUM1
-
 Arabidopsis thaliana
FUM2
-
 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
 fumarate hydratase reaction Arabidopsis thaliana
8
-
-
 Arabidopsis thaliana
8.2
-
 malate dehydratase reaction Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8.5
-
 L-malate pH 8.0, 30°C Arabidopsis thaliana
8.5
-
 fumarate presence of 3 mM oxalacetate, pH 8.0, 30°C Arabidopsis thaliana
8.7
-
 L-malate pH 8.2, 30°C Arabidopsis thaliana
9
-
 L-malate pH 8.2, 30°C Arabidopsis thaliana
9.7
-
 fumarate presence of 3 mM oxaloacetate, pH 8.0, 30°C Arabidopsis thaliana
30
-
 fumarate pH 8.0, 30°C Arabidopsis thaliana
36.6
-
 fumarate pH 7.7, 30°C Arabidopsis thaliana
37
-
 L-malate presence of 3 mM oxalacetate, pH 8.0, 30°C Arabidopsis thaliana
37.8
-
 L-malate presence of 3 mM oxaloacetate, pH 8.0, 30°C Arabidopsis thaliana
57.6
-
 fumarate pH 7.7, 30°C Arabidopsis thaliana