KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
S-malate | pH 6.5 cytosolic form, Vmax: 4.5 micromol/min/mg, temperature not specified in the publication | Zea mays | |
0.05 | - |
S-malate | pH 7 mitochondrial, Vmax: 0.3 micromol/min/mg, temperature not specified in the publication | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Zea mays | 5829 | - |
mitochondrion | - |
Zea mays | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | cytosolic form gets strongly activated, mitochondrial form only moderately activated | Zea mays | |
Mn2+ | cytosolic form gets strongly activated, mitochondrial form only less effectively activated | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using fractionation by (NH4)2SO4, gel filtration of Sephadex G-25 and ion exchange chromatography of DEAE cellulose | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | localisation is only mitochondrial | Zea mays | - |
scutellum | highest fumarase activity detected | Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-malate | - |
Zea mays | fumarate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
fumarase | - |
Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
cytosolic form | Zea mays |
7 | - |
mitochondrial form | Zea mays |