Literature summary for 4.2.1.2 extracted from

Shimoyama, T.; Rajashekhara, E.; Ohmori, D.; Kosaka, T.; Watanabe, K.
MmcBC in Pelotomaculum thermopropionicum represents a novel group of prokaryotic fumarases (2007), FEMS Microbiol. Lett., 270, 207-213.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21	Pelotomaculum thermopropionicum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.43	-	fumarate	pH 7.0, 55°C	Pelotomaculum thermopropionicum
0.59	-	(S)-malate	pH 7.0, 55°C	Pelotomaculum thermopropionicum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe	enzyme contains an oxygen-sensitive [4Fe-4S] cluster	Pelotomaculum thermopropionicum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
64200	-	gel filtration	Pelotomaculum thermopropionicum

Organism

Organism	UniProt	Comment	Textmining
Pelotomaculum thermopropionicum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pelotomaculum thermopropionicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
45	-	at 55°C	Pelotomaculum thermopropionicum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-malate	-	Pelotomaculum thermopropionicum	fumarate + H2O	-	r

Subunits

Subunits	Comment	Organism
dimer	-	Pelotomaculum thermopropionicum

Synonyms

Synonyms	Comment	Organism
MmcBC	-	Pelotomaculum thermopropionicum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
70	-	-	Pelotomaculum thermopropionicum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
25.2	-	(S)-malate	pH 7.0, 55°C	Pelotomaculum thermopropionicum
219	-	fumarate	pH 7.0, 55°C	Pelotomaculum thermopropionicum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Pelotomaculum thermopropionicum

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Release 2023.1 (January 2023)