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Literature summary for 4.2.1.2 extracted from

  • Genda, T.; Watabe, S.; Ozaki, H.
    Purification and characterization of fumarase from Corynebacterium glutamicum (2006), Biosci. Biotechnol. Biochem., 70, 1102-1109.
    View publication on PubMed
Search for more literature for 4.2.1.2

General Stability

General Stability Organism
dithiothreitol partially restores from urea and alkaline inactivation Corynebacterium glutamicum

Inhibitors

Inhibitors Comment Organism Structure
(R)-malate mixed-type inhibition Corynebacterium glutamicum
ATP competitive or mixed-type inhibition Corynebacterium glutamicum
D-Tartrate competitive Corynebacterium glutamicum
meso-tartrate competitive Corynebacterium glutamicum
additional information no inactivation with L-tartrate, citrate, succinate, glycine, maleate, 1 mM iodoacetate, 1 mM iodoacetamide, 1 mM N-ethylmaleimide or 0.5 mM PCMB Corynebacterium glutamicum
pyromellitate competitive Corynebacterium glutamicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.38
-
 fumarate MES-NaOH buffer, pH 7.0 Corynebacterium glutamicum
0.67
-
 fumarate MES-NaOH buffer, pH 7.0 Corynebacterium glutamicum
0.81
-
 fumarate MES-NaOH buffer, pH 6.0 Corynebacterium glutamicum
1.4
-
 (S)-malate MES-NaOH buffer, pH 6.0 Corynebacterium glutamicum
1.8
-
 (S)-malate MES-NaOH buffer, pH 7.0 Corynebacterium glutamicum
1.8
-
 (S)-malate phosphate buffer, pH 6.0 Corynebacterium glutamicum
3.1
-
 fumarate phosphate buffer, pH 6.0 Corynebacterium glutamicum
3.8
-
 fumarate phosphate buffer, pH 7.0 Corynebacterium glutamicum
4.2
-
 fumarate phosphate buffer, pH 6.0 Corynebacterium glutamicum
5
-
 (S)-malate MES-NaOH buffer, pH 7.0 Corynebacterium glutamicum
6.3
-
 (S)-malate phosphate buffer, pH 7.0 Corynebacterium glutamicum
13
-
 (S)-malate phosphate buffer, pH 6.0 Corynebacterium glutamicum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 2 mM, slight stimulation Corynebacterium glutamicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 4 * 50000, SDS-PAGE Corynebacterium glutamicum
200000
-
 gel filtration Corynebacterium glutamicum

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum Q8NRN8 ATCC 14067
-

Purification (Commentary)

Purification (Comment) Organism
-
 Corynebacterium glutamicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
315
-
-
 Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate
-
 Corynebacterium glutamicum fumarate + H2O
-
 r

Subunits

Subunits Comment Organism
tetramer 4 * 50000, SDS-PAGE Corynebacterium glutamicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
21
-
 pH 7.6, stable Corynebacterium glutamicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
34
-
 (S)-malate MES-NaOH buffer, pH 6.0 Corynebacterium glutamicum
48
-
 (S)-malate phosphate buffer, pH 6.0 Corynebacterium glutamicum
110
-
 (S)-malate MES-NaOH buffer, pH 7.0 Corynebacterium glutamicum
200
-
 (S)-malate phosphate buffer, pH 7.0 Corynebacterium glutamicum
290
-
 (S)-malate MES-NaOH buffer, pH 7.0 Corynebacterium glutamicum
310
-
 fumarate MES-NaOH buffer, pH 6.0 Corynebacterium glutamicum
430
-
 fumarate MES-NaOH buffer, pH 7.0 Corynebacterium glutamicum
460
-
 (S)-malate phosphate buffer, pH 6.0 Corynebacterium glutamicum
510
-
 fumarate phosphate buffer, pH 6.0 Corynebacterium glutamicum
650
-
 fumarate MES-NaOH buffer, pH 7.0 Corynebacterium glutamicum
720
-
 fumarate phosphate buffer, pH 6.0 Corynebacterium glutamicum
730
-
 fumarate phosphate buffer, pH 7.0 Corynebacterium glutamicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 phosphate buffer, substrate: fumarate Corynebacterium glutamicum
7.5
-
 Tris-HCl buffer, substrate: fumarate Corynebacterium glutamicum
8.3
-
 phosphate buffer, substrate: malate Corynebacterium glutamicum
8.5
-
 Tris-HCl buffer, substrate: malate Corynebacterium glutamicum

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 stable Corynebacterium glutamicum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.11
-
 meso-tartrate
-
 Corynebacterium glutamicum
1.3
-
 pyromellitate
-
 Corynebacterium glutamicum
2.5
-
 (R)-malate
-
 Corynebacterium glutamicum
16
-
 D-Tartrate
-
 Corynebacterium glutamicum