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Literature summary for 4.2.1.2 extracted from

  • Puchegger, S.; Redl, B.; Stöpfler, G.
    Purification and properties of a thermostable fumarate hydratase from the archaeobacterium Sulfolobus solfataricus (1990), J. Gen. Microbiol., 136, 1537-1541.
    View publication on PubMed

General Stability

General Stability Organism
50% v/v ethanol, 50% v/v methanol, 50% v/v 2-propanol, 4 M urea or 0.1% SDS, stable after treatment for 10 h at room temperature Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
2-propanol 15-20% of maximum activity when assayed in 40% (v/v) 2-propanol Saccharolobus solfataricus
ethanol 15-20% of maximum activity when assayed in 40% (v/v) ethanol Saccharolobus solfataricus
K3PO4 10 mM Saccharolobus solfataricus
methanol 94% of maximum activity when assayed in 40% (v/v) methanol Saccharolobus solfataricus
phosphate
-
Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.125
-
fumarate
-
Saccharolobus solfataricus
0.125
-
fumarate pH 7.5, 70°C Saccharolobus solfataricus
0.3
-
(S)-malate
-
Saccharolobus solfataricus
0.3
-
(S)-malate pH 7.5, 70°C Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
4 * 45000, SDS-PAGE Saccharolobus solfataricus
160000
-
sucrose density gradient centrifugation Saccharolobus solfataricus
170000
-
gel filtration Saccharolobus solfataricus

Organic Solvent Stability

Organic Solvent Comment Organism
2-propanol 50% (v/v), 10 h at room temperature, no significant loss of activity, slight loss of activity after 14 days Saccharolobus solfataricus
Ethanol 50% (v/v), 10 h at room temperature, no significant loss of activity, slight loss of activity after 14 days Saccharolobus solfataricus
Methanol 50% (v/v), 10 h at room temperature, no significant loss of activity, slight loss of activity after 14 days Saccharolobus solfataricus
SDS 0.1% (v/v), 10 h at room temperature, no significant loss of activity, slight loss of activity after 14 days Saccharolobus solfataricus
urea 0.1% (v/v), 10 h at room temperature, no significant loss of activity, slight loss of activity after 14 days Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus
-
DSM 1616
-
Saccharolobus solfataricus DSM 1616
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
205
-
-
Saccharolobus solfataricus
205
-
pH 7.5, 70°C Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate
-
Saccharolobus solfataricus fumarate + H2O
-
r
(S)-malate
-
Saccharolobus solfataricus DSM 1616 fumarate + H2O
-
r
fumarate + H2O
-
Saccharolobus solfataricus L-malate
-
r
fumarate + H2O
-
Saccharolobus solfataricus DSM 1616 L-malate
-
r
additional information no activity with D-malate, maleate, citramalate and citrate Saccharolobus solfataricus ?
-
?
additional information no activity with D-malate, maleate, citramalate and citrate Saccharolobus solfataricus DSM 1616 ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 45000, SDS-PAGE Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
assay at Saccharolobus solfataricus
85
-
-
Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
85 95 inactive at 40°C, 85°C: optimum, 95°C: 66% of maximal activity Saccharolobus solfataricus
85 95 85°C: maximal activity, 95°C: 66% of maximal activity, the enzyme is almost inactive at 40°C Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
1 h, stable Saccharolobus solfataricus
85
-
1 h, stable Saccharolobus solfataricus
90
-
30 min, 72% of maximal activity Saccharolobus solfataricus
90
-
30 min, 72% loss of activity Saccharolobus solfataricus
100
-
30 min, complete loss of activity Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Saccharolobus solfataricus
8
-
-
Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 pH 5.5: 43% of maximal activity, pH 9.0: 70% of maximal activity Saccharolobus solfataricus

pI Value

Organism Comment pI Value Maximum pI Value
Saccharolobus solfataricus isoelectric focusing
-
7.1