Literature summary for 4.2.1.2 extracted from

Van Kuijk, B.L.M.; van Loo, N.D.; Arendsen, A.F.; Hagen, W.R.; Stams, A.J.M.
Purification and characterization of fumarase from the syntrophic propionate-oxidizing bacterium strain MPOB (1996), Arch. Microbiol., 165, 126-131.

Search for more literature for 4.2.1.2

General Stability

General Stability	Organism
exposure to air at room temperature causes 50% loss of activity, reactivation with FeSO4 and 2-mercaptoethanol	Bacteria

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.25	-	fumarate	-	Bacteria
2.38	-	L-malate	-	Bacteria

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	2 * 60000, SDS-PAGE	Bacteria
114000	-	gel filtration	Bacteria

Organism

Organism	UniProt	Comment	Textmining
Bacteria	-	synthrophic propionate-oxidizing bacterium strain MPOB	-

Purification (Commentary)

Purification (Comment)	Organism
-	Bacteria

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
971	-	-	Bacteria

Storage Stability

Storage Stability	Organism
4°C, under N2, stable for at least 1 month	Bacteria

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-malate	-	Bacteria	fumarate + H2O	-	r
fumarate + H2O	-	Bacteria	L-malate	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 60000, SDS-PAGE	Bacteria

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
53	55	-	Bacteria

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
540	-	(S)-malate	-	Bacteria
690	-	fumarate	-	Bacteria

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Bacteria

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	11.5	no activity below pH 5.5 and above pH 11.5	Bacteria

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Release 2023.1 (January 2023)