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Literature summary for 4.2.1.2 extracted from
- Purification and catalytic properties of "thermostable" fumarase from Bacillus stearothermophilus NU-10 and Thermus X-1 (1976), Experientia, 26, 207-222.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no substrate inhibition by high levels of malate | Geobacillus stearothermophilus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 180000 | - |
gel filtration | Geobacillus stearothermophilus |
| 180000 | - |
gel filtration | Thermus sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
NU-10 | - |
| Geobacillus stearothermophilus NU-10 | - |
NU-10 | - |
| Thermus sp. | - |
X-1 | - |
| Thermus sp. X-1 | - |
X-1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
- |
Thermus sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate | - |
Geobacillus stearothermophilus | fumarate + H2O | - |
? |  |
| (S)-malate | - |
Thermus sp. | fumarate + H2O | - |
? | |
| (S)-malate | - |
Thermus sp. X-1 | fumarate + H2O | - |
? | |
| (S)-malate | - |
Geobacillus stearothermophilus NU-10 | fumarate + H2O | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 81 | - |
- |
Geobacillus stearothermophilus |
| 83 | - |
- |
Thermus sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Geobacillus stearothermophilus |
| 8 | - |
- |
Thermus sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | pH 7: about 60% of maximal activity, pH 10: about 30% of maximal activity | Geobacillus stearothermophilus |
| 7 | 10 | pH 7: about 60% of maximal activity, pH 10: about 30% of maximal activity | Thermus sp. |
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