Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Myxococcus xanthus |
Crystallization (Comment) | Organism |
---|---|
structures refined to 2.05 and 1.1 A, in the apo form and bound to coenzyme A, respectively. The dehydration of 3-hydroxy-3-methylglutaryl CoA to 3-methylglutaconyl CoA involves Glu112 and Glu132 and likely employs the typical crotonase acid-base mechanism. Residues Tyr231 and Arg69 are key players in positioning the substrate to enable catalysis | Myxococcus xanthus |
Protein Variants | Comment | Organism |
---|---|---|
E112Q/E132Q/R69A | protein is insoluble | Myxococcus xanthus |
E112Q/E132Q/R69A/Y231F | protein is insoluble | Myxococcus xanthus |
E112Q/E132Q/Y231F | protein is insoluble | Myxococcus xanthus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Myxococcus xanthus | Q1D5Y4 | - |
- |
Myxococcus xanthus DK 1622 | Q1D5Y4 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
LiuC | - |
Myxococcus xanthus |