Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.179 extracted from

  • Matsuyama, T.; Tanaka, K.
    On the enzyme of Aspergillus fumigatus producing difructose anhydride I from inulobiose (1989), Agric. Biol. Chem., 53, 831-832 .
No PubMed abstract available
Search for more literature for 4.2.1.179

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 39% inhibition at 2.5 mM Aspergillus fumigatus
Cu2+ 67% inhibition at 2.5 mM Aspergillus fumigatus
dithiothreitol 14% inhibition at 2.5 mM Aspergillus fumigatus
L-cysteine 23% inhibition at 2.5 mM Aspergillus fumigatus
Zn2+ 36% inhibition at 2.5 mM Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
241
-
 bis-D-fructose 2',1:2,1'-dianhydride at pH 6.0 and 37°C Aspergillus fumigatus
377
-
 Inulobiose at pH 6.0 and 37°C Aspergillus fumigatus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Ca2+, Mg2+, and Mn2+ have no effect on the enzyme activity Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
bis-D-fructose 2',1:2,1'-dianhydride + H2O Aspergillus fumigatus i.e. difructose anhydride I inulobiose
-
 r
inulobiose Aspergillus fumigatus
-
 bis-D-fructose 2',1:2,1'-dianhydride + H2O
-
 r

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
-
-

Storage Stability

Storage Stability Organism
4°C, at pH 6.0 under toluene, several months, no loss of activity Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bis-D-fructose 2',1:2,1'-dianhydride + H2O i.e. difructose anhydride I Aspergillus fumigatus inulobiose
-
 r
bis-D-fructose 2',1:2,1'-dianhydride + H2O the equilibrium of the enzymic reaction is at about 85% in the cyclization and about 15% in the hydrolysis Aspergillus fumigatus inulobiose
-
 r
inulobiose
-
 Aspergillus fumigatus bis-D-fructose 2',1:2,1'-dianhydride + H2O
-
 r
inulobiose the equilibrium of the enzymic reaction is at about 85% in the cyclization and about 15% in the hydrolysis Aspergillus fumigatus bis-D-fructose 2',1:2,1'-dianhydride + H2O
-
 r

Synonyms

Synonyms Comment Organism
difructose anhydride I hydrolase
-
 Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
48
-
-
 Aspergillus fumigatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 the enzyme is stable up to 50°C at pH 6.0, with Tris-malate buffer Aspergillus fumigatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Aspergillus fumigatus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 the enzyme is stable from pH 6.0 to 8.6 at 37°C Aspergillus fumigatus