KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.5 | - |
alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride | at pH 6.0 and 45°C | Arthrobacter sp. H65-7 | |
30.9 | - |
Inulobiose | at pH 6.0 and 45°C | Arthrobacter sp. H65-7 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
125000 | - |
gel filtration | Arthrobacter sp. H65-7 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride + H2O | Arthrobacter sp. H65-7 | highest activity | inulobiose | - |
r | |
D-fructose + H2O | Arthrobacter sp. H65-7 | very low activity | ? | - |
? | |
inulin + H2O | Arthrobacter sp. H65-7 | high activity | ? | - |
? | |
inulobiose | Arthrobacter sp. H65-7 | low activity | alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride + H2O | - |
r | |
levan + H2O | Arthrobacter sp. H65-7 | very low activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. H65-7 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and chromatographies on DEAE-Toyopearl 650M, butyl-Sepharose 4B, TSKgel G3000SW and Biophoresis III | Arthrobacter sp. H65-7 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.53 | - |
cell-free extract, at pH 6.0 and 45°C | Arthrobacter sp. H65-7 |
19.2 | - |
purified enzyme, at pH 6.0 and 45°C | Arthrobacter sp. H65-7 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride + H2O | highest activity | Arthrobacter sp. H65-7 | inulobiose | - |
r | |
D-fructose + H2O | very low activity | Arthrobacter sp. H65-7 | ? | - |
? | |
inulin + H2O | high activity | Arthrobacter sp. H65-7 | ? | - |
? | |
inulobiose | low activity | Arthrobacter sp. H65-7 | alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride + H2O | - |
r | |
levan + H2O | very low activity | Arthrobacter sp. H65-7 | ? | - |
? | |
additional information | no activity with di-D-fructofuranose 6,2' : 2,6'-dianhydride, levanbiose, xylan, starch, cellulose, raffinose, sucrose, maltose, lactose, cellobiose, D-glucose, D-xylose, glycerol, and acetate | Arthrobacter sp. H65-7 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 61000, SDS-PAGE | Arthrobacter sp. H65-7 |
Synonyms | Comment | Organism |
---|---|---|
DFA III hydrolysis enzyme | - |
Arthrobacter sp. H65-7 |
DFA IIIase | - |
Arthrobacter sp. H65-7 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Arthrobacter sp. H65-7 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 75 | the enzyme is stable up to 60°C with more than 80% of the enzyme activity remaining after 15 min. More than 80% of the activity is kept up to 75°C in the cell-free extracts | Arthrobacter sp. H65-7 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Arthrobacter sp. H65-7 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 10 | more than 80% of the enzyme activity remains between pH 4.5 and 10.0 after 18 h incubation | Arthrobacter sp. H65-7 |