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Literature summary for 4.2.1.178 extracted from

  • Sakurai, H.; Yokota, A.; Sumita, Y.; Mori, Y.; Matsui, H.; Tomita, F.
    Metabolism of DFA III by Arthrobacter sp. H65-7 Purification and properties of a DFA III hydrolysis enzyme (DFA IIIase) (1997), Biosci. Biotechnol. Biochem., 61, 989-993 .
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
12.5
-
alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride at pH 6.0 and 45°C Arthrobacter sp. H65-7
30.9
-
Inulobiose at pH 6.0 and 45°C Arthrobacter sp. H65-7

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
125000
-
gel filtration Arthrobacter sp. H65-7

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride + H2O Arthrobacter sp. H65-7 highest activity inulobiose
-
r
D-fructose + H2O Arthrobacter sp. H65-7 very low activity ?
-
?
inulin + H2O Arthrobacter sp. H65-7 high activity ?
-
?
inulobiose Arthrobacter sp. H65-7 low activity alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride + H2O
-
r
levan + H2O Arthrobacter sp. H65-7 very low activity ?
-
?

Organism

Organism UniProt Comment Textmining
Arthrobacter sp. H65-7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and chromatographies on DEAE-Toyopearl 650M, butyl-Sepharose 4B, TSKgel G3000SW and Biophoresis III Arthrobacter sp. H65-7

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.53
-
cell-free extract, at pH 6.0 and 45°C Arthrobacter sp. H65-7
19.2
-
purified enzyme, at pH 6.0 and 45°C Arthrobacter sp. H65-7

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride + H2O highest activity Arthrobacter sp. H65-7 inulobiose
-
r
D-fructose + H2O very low activity Arthrobacter sp. H65-7 ?
-
?
inulin + H2O high activity Arthrobacter sp. H65-7 ?
-
?
inulobiose low activity Arthrobacter sp. H65-7 alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride + H2O
-
r
levan + H2O very low activity Arthrobacter sp. H65-7 ?
-
?
additional information no activity with di-D-fructofuranose 6,2' : 2,6'-dianhydride, levanbiose, xylan, starch, cellulose, raffinose, sucrose, maltose, lactose, cellobiose, D-glucose, D-xylose, glycerol, and acetate Arthrobacter sp. H65-7 ?
-
-

Subunits

Subunits Comment Organism
homodimer 2 * 61000, SDS-PAGE Arthrobacter sp. H65-7

Synonyms

Synonyms Comment Organism
DFA III hydrolysis enzyme
-
Arthrobacter sp. H65-7
DFA IIIase
-
Arthrobacter sp. H65-7

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Arthrobacter sp. H65-7

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 75 the enzyme is stable up to 60°C with more than 80% of the enzyme activity remaining after 15 min. More than 80% of the activity is kept up to 75°C in the cell-free extracts Arthrobacter sp. H65-7

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Arthrobacter sp. H65-7

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 10 more than 80% of the enzyme activity remains between pH 4.5 and 10.0 after 18 h incubation Arthrobacter sp. H65-7