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Literature summary for 4.2.1.178 extracted from
- Formation of inulobiose from difructoseanhydride III catalysed by a lysate from Arthrobacter ureafaciens ATCC 21124 (2000), Biocatal. Biotransform., 18, 443-455 .
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| product formation can be enhanced by buffering with 0.1 M Na-borate/HCl buffer | Paenarthrobacter ureafaciens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| difructoseanhydride III | inulobiose formation is slightly inhibited at difructoseanhydride concentrations of more than 75-100g/l (0.23-0.31 M) | Paenarthrobacter ureafaciens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 22 | - |
Inulobiose | at pH 5.5 and 60°C | Paenarthrobacter ureafaciens |  |
| 31 | - |
alpha-D-fructofuranose-beta-D-fructofuranose-1,2':2,3'-dianhydride | at pH 5.5 and 60°C | Paenarthrobacter ureafaciens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-fructofuranose-beta-D-fructofuranose-1,2':2,3'-dianhydride + H2O | Paenarthrobacter ureafaciens | - |
inulobiose | - |
r | |
| alpha-D-fructofuranose-beta-D-fructofuranose-1,2':2,3'-dianhydride + H2O | Paenarthrobacter ureafaciens ATCC 21124 | - |
inulobiose | - |
r | |
| inulobiose | Paenarthrobacter ureafaciens | - |
alpha-D-fructofuranose-beta-D-fructofuranose-1,2':2,3'-dianhydride + H2O | - |
r | |
| inulobiose | Paenarthrobacter ureafaciens ATCC 21124 | - |
alpha-D-fructofuranose-beta-D-fructofuranose-1,2':2,3'-dianhydride + H2O | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenarthrobacter ureafaciens | - |
- |
- |
| Paenarthrobacter ureafaciens ATCC 21124 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-fructofuranose-beta-D-fructofuranose-1,2':2,3'-dianhydride + H2O | - |
Paenarthrobacter ureafaciens | inulobiose | - |
r | |
| alpha-D-fructofuranose-beta-D-fructofuranose-1,2':2,3'-dianhydride + H2O | - |
Paenarthrobacter ureafaciens ATCC 21124 | inulobiose | - |
r | |
| inulobiose | - |
Paenarthrobacter ureafaciens | alpha-D-fructofuranose-beta-D-fructofuranose-1,2':2,3'-dianhydride + H2O | - |
r | |
| inulobiose | - |
Paenarthrobacter ureafaciens ATCC 21124 | alpha-D-fructofuranose-beta-D-fructofuranose-1,2':2,3'-dianhydride + H2O | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DFA-IIIase | - |
Paenarthrobacter ureafaciens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Paenarthrobacter ureafaciens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | the enzyme shows about 70% activity after 10 h at 30°C. The enzyme shows about 70% activity after 6 h at 40°C. The enzyme shows about 50% activity after 6 h at 50°C. The enzyme shows about 50% activity after 3 h at 60°C. The enzyme is inactive after 24 h incubation at 30-60°C | Paenarthrobacter ureafaciens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Paenarthrobacter ureafaciens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | the enzyme shows high stability in the range of pH 5.0-9.0 retaining more than 80% activity after 24 h incubation | Paenarthrobacter ureafaciens |
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