Literature summary for 4.2.1.172 extracted from

Levin, B.J.; Huang, Y.Y.; Peck, S.C.; Wei, Y.; Martinez-Del Campo, A.; Marks, J.A.; Franzosa, E.A.; Huttenhower, C.; Balskus, E.P.
A prominent glycyl radical enzyme in human gut microbiomes metabolizes trans-4-hydroxy-L-proline (2017), Science, 355, eaai8386.

Search for more literature for 4.2.1.172

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Clostridioides difficile

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.2	-	trans-4-hydroxy-L-proline	pH not specified in the publication, temperature not specified in the publication	Clostridioides difficile

Organism

Organism	UniProt	Comment	Textmining
Clostridioides difficile	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	enzyme displays undetectable or greatly reduced activity toward other hydroxyproline stereoisomers	Clostridioides difficile	?	-	-
trans-4-hydroxy-L-proline	-	Clostridioides difficile	(S)-1-pyrroline-5-carboxylate + H2O	-	?

Synonyms

Synonyms	Comment	Organism
t4LHypD	-	Clostridioides difficile

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
45	-	trans-4-hydroxy-L-proline	pH not specified in the publication, temperature not specified in the publication	Clostridioides difficile

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
38	-	trans-4-hydroxy-L-proline	pH not specified in the publication, temperature not specified in the publication	Clostridioides difficile

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Release 2023.1 (January 2023)