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Literature summary for 4.2.1.171 extracted from

  • Zhang, X.; Kumar, R.; Vetting, M.; Zhao, S.; Jacobson, M.; Almo, S.; Gerlt, J.
    A unique cis-3-hydroxy-L-proline dehydratase in the enolase superfamily (2015), J. Am. Chem. Soc., 137, 1388-1391.
    View publication on PubMed
Search for more literature for 4.2.1.171

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with Mg2+, to 2.2 A resolution Roseibium aggregatum

Protein Variants

Protein Variants Comment Organism
K165A mutation in general acid/base residue, complete loss of activity Roseibium aggregatum
K265A mutation in general acid/base residue, complete loss of activity Roseibium aggregatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8.1
-
 cis-3-hydroxy-L-proline pH 8.0, temperature not specified in the publication Roseibium aggregatum

Organism

Organism UniProt Comment Textmining
Roseibium aggregatum A0NXQ8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-3-hydroxy-L-proline
-
 Roseibium aggregatum 1-pyrroline-2-carboxylate + H2O
-
 ?
additional information the enzyme catalyzes the competing 2-epimerization to trans-3-hydroxy-D-proline (1,1-proton transfer) and dehydration to 1-pyrroline-2-carboxylate (beta-elimination), with eventual total dehydration. The reactions are initiated by abstraction of the alpha-proton of the substrate. The rate of epimerization is about 1.5fold greater than that of dehydration at substrate concentrations both below and above the value of the KM. No substrates: L-proline and stereoisomers of 3-hydroxyproline, 4-hydroxyproline and their mono- and di-N-methylated derivatives Roseibium aggregatum ?
-
 ?

Synonyms

Synonyms Comment Organism
SIAM614_28497
-
 Roseibium aggregatum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
23
-
 cis-3-hydroxy-L-proline pH 8.0, temperature not specified in the publication Roseibium aggregatum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.8
-
 cis-3-hydroxy-L-proline pH 8.0, temperature not specified in the publication Roseibium aggregatum