Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.17 extracted from

  • Hawkins, A.B.; Adams, M.W.; Kelly, R.M.
    Conversion of 4-hydroxybutyrate to acetyl coenzyme A and its anapleurosis in the Metallosphaera sedula 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway (2014), Appl. Environ. Microbiol., 80, 2536-2545.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.17

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2 (DE3) cells Metallosphaera sedula

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 (S)-3-hydroxybutyryl-CoA at pH 7.0 and 70°C Metallosphaera sedula
0.07
-
 crotonoyl-CoA at pH 7.0 and 70°C Metallosphaera sedula

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-3-hydroxybutyryl-CoA Metallosphaera sedula
-
 crotonoyl-CoA + H2O
-
 r
(S)-3-hydroxybutyryl-CoA Metallosphaera sedula DSM 5348
-
 crotonoyl-CoA + H2O
-
 r
crotonoyl-CoA + H2O Metallosphaera sedula
-
 (S)-3-hydroxybutyryl-CoA
-
 r
crotonoyl-CoA + H2O Metallosphaera sedula DSM 5348
-
 (S)-3-hydroxybutyryl-CoA
-
 r

Organism

Organism UniProt Comment Textmining
Metallosphaera sedula
-
-
-
Metallosphaera sedula DSM 5348
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography and Superdex 200 gel filtration Metallosphaera sedula

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-3-hydroxybutyryl-CoA
-
 Metallosphaera sedula crotonoyl-CoA + H2O
-
 r
(S)-3-hydroxybutyryl-CoA
-
 Metallosphaera sedula DSM 5348 crotonoyl-CoA + H2O
-
 r
crotonoyl-CoA + H2O
-
 Metallosphaera sedula (S)-3-hydroxybutyryl-CoA
-
 r
crotonoyl-CoA + H2O
-
 Metallosphaera sedula DSM 5348 (S)-3-hydroxybutyryl-CoA
-
 r

Synonyms

Synonyms Comment Organism
CCH/HBCD
-
 Metallosphaera sedula
crotonyl-CoA hydratase
-
 Metallosphaera sedula

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15
-
 (S)-3-hydroxybutyryl-CoA at pH 7.0 and 70°C Metallosphaera sedula
19
-
 crotonoyl-CoA at pH 7.0 and 70°C Metallosphaera sedula

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Metallosphaera sedula

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
260
-
 crotonoyl-CoA at pH 7.0 and 70°C Metallosphaera sedula
260
-
 (S)-3-hydroxybutyryl-CoA at pH 7.0 and 70°C Metallosphaera sedula