Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2 (DE3) cells | Metallosphaera sedula |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
(S)-3-hydroxybutyryl-CoA | at pH 7.0 and 70°C | Metallosphaera sedula | |
0.07 | - |
crotonoyl-CoA | at pH 7.0 and 70°C | Metallosphaera sedula |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxybutyryl-CoA | Metallosphaera sedula | - |
crotonoyl-CoA + H2O | - |
r | |
(S)-3-hydroxybutyryl-CoA | Metallosphaera sedula DSM 5348 | - |
crotonoyl-CoA + H2O | - |
r | |
crotonoyl-CoA + H2O | Metallosphaera sedula | - |
(S)-3-hydroxybutyryl-CoA | - |
r | |
crotonoyl-CoA + H2O | Metallosphaera sedula DSM 5348 | - |
(S)-3-hydroxybutyryl-CoA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Metallosphaera sedula | - |
- |
- |
Metallosphaera sedula DSM 5348 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography and Superdex 200 gel filtration | Metallosphaera sedula |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxybutyryl-CoA | - |
Metallosphaera sedula | crotonoyl-CoA + H2O | - |
r | |
(S)-3-hydroxybutyryl-CoA | - |
Metallosphaera sedula DSM 5348 | crotonoyl-CoA + H2O | - |
r | |
crotonoyl-CoA + H2O | - |
Metallosphaera sedula | (S)-3-hydroxybutyryl-CoA | - |
r | |
crotonoyl-CoA + H2O | - |
Metallosphaera sedula DSM 5348 | (S)-3-hydroxybutyryl-CoA | - |
r |
Synonyms | Comment | Organism |
---|---|---|
CCH/HBCD | - |
Metallosphaera sedula |
crotonyl-CoA hydratase | - |
Metallosphaera sedula |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
(S)-3-hydroxybutyryl-CoA | at pH 7.0 and 70°C | Metallosphaera sedula | |
19 | - |
crotonoyl-CoA | at pH 7.0 and 70°C | Metallosphaera sedula |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Metallosphaera sedula |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
260 | - |
crotonoyl-CoA | at pH 7.0 and 70°C | Metallosphaera sedula | |
260 | - |
(S)-3-hydroxybutyryl-CoA | at pH 7.0 and 70°C | Metallosphaera sedula |