Cloned (Comment) | Organism |
---|---|
expression of a His6-tagged truncated ECH2 mutant enzyme | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-methylenecyclopropylformyl-CoA | methylenecyclopropylformyl-CoA is a better inhibitor for enoyl-CoA hydratase 2 than for enoyl-CoA hydratase 1 | Rattus norvegicus | |
(S)-methylenecyclopropylformyl-CoA | methylenecyclopropylformyl-CoA is a better inhibitor for enoyl-CoA hydratase 2 than for enoyl-CoA hydratase 1 | Rattus norvegicus | |
3-octynoyl-CoA | irreversibly inactivates only enoyl-CoA hydratase 2, the catalytic residue Glu47 is covalently labeled by the inhibitor | Rattus norvegicus | |
methylenecyclopropylformyl-CoA | a metabolite derived from a natural amino acid, (methylenecyclopropyl)glycine, that inactivates enoyl-CoA hydratase 1 and enoyl-CoA hydratase 2. Competence of (R)- and (S)-MCPF-CoA to inactivate the ECH2 and kinetic analysis, enzyme-inhibitor complex structures, mass spectrometric analysis, inhibition mechanism, overview | Rattus norvegicus | |
additional information | 3-octynoyl-CoA is not an inhibitor of enoyl-CoA hydratase 1 | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | In eukaryotes, ECH2 is a 31 kDa integral part of multifunctional protein-2, MFP-2, also called multifunctional enzyme 2, D-bifunctional enzyme, or 17-beta-estradiol dehydrogenase type IV. The MFP-2 plays a central role in peroxisomal beta-oxidation as it handles most peroxisomal beta-oxidation substrates | ? | - |
? | |
additional information | Rattus norvegicus | the beta-oxidation in mitochondria involves a (3S)-hydroxyacyl-CoA intermediate, while the beta-oxidation in peroxisomes has a (3R)-hydroxyacyl-CoA intermediate. The enzymes responsible for the formation of these two different intermediates are enoyl-CoA hydratase 1 (ECH1) in mitochondria and enoyl-CoA hydratase 2 (ECH2) in peroxisomes | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged truncated ECH2 mutant enzyme | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-octynoyl-CoA + H2O | reaction of ECH1, ECH2 is inactivated by the compound, it is possible that 3-octynoyl-CoA is isomerized to reactive 2,3-octadienoyl-CoA, overview | Rattus norvegicus | 3-ketooctanoyl-CoA | - |
? | |
additional information | In eukaryotes, ECH2 is a 31 kDa integral part of multifunctional protein-2, MFP-2, also called multifunctional enzyme 2, D-bifunctional enzyme, or 17-beta-estradiol dehydrogenase type IV. The MFP-2 plays a central role in peroxisomal beta-oxidation as it handles most peroxisomal beta-oxidation substrates | Rattus norvegicus | ? | - |
? | |
additional information | the beta-oxidation in mitochondria involves a (3S)-hydroxyacyl-CoA intermediate, while the beta-oxidation in peroxisomes has a (3R)-hydroxyacyl-CoA intermediate. The enzymes responsible for the formation of these two different intermediates are enoyl-CoA hydratase 1 (ECH1) in mitochondria and enoyl-CoA hydratase 2 (ECH2) in peroxisomes | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | mitochondrial ECH1 that exists as a hexamer | Rattus norvegicus |
homodimer | ECH2 exists as a homodimer in its crystal structure | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
ECH1 | - |
Rattus norvegicus |
ECH2 | - |
Rattus norvegicus |
enoyl-CoA hydratase 1 | - |
Rattus norvegicus |
enoyl-CoA hydratase 2 | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.041 | - |
(R)-methylenecyclopropylformyl-CoA | 25°C, ECH2 | Rattus norvegicus | |
0.047 | - |
(R)-methylenecyclopropylformyl-CoA | 25°C, ECH1 | Rattus norvegicus | |
0.049 | - |
(R)-methylenecyclopropylformyl-CoA | 25°C, ECH1 | Rattus norvegicus | |
0.0492 | - |
(R)-methylenecyclopropylformyl-CoA | 25°C | Rattus norvegicus | |
0.053 | - |
(S)-methylenecyclopropylformyl-CoA | 25°C, ECH2 | Rattus norvegicus | |
0.0571 | - |
(S)-methylenecyclopropylformyl-CoA | 25°C | Rattus norvegicus | |
0.065 | - |
3-octynoyl-CoA | ECH2 | Rattus norvegicus |