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Literature summary for 4.2.1.17 extracted from

  • Waterson, R.M.; Hill, R.L.
    Enoyl coenzyme A hydratase (crotonase). Catalytic properties of crotonase and its possible regulatory role in fatty acid oxidation (1972), J. Biol. Chem., 247, 5258-5265.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
acetoacetyl-CoA enolate form of acetoacetyl-CoA acts as a competitive inhibitor. 6 molecules of inhibitor are bound per molecule of enzyme Bos taurus
decenoyl-CoA
-
Bos taurus
hexadecenoyl-CoA
-
Bos taurus
hexenoyl-CoA
-
Bos taurus
additional information no inhibition: ethyl acetoacetate, acetoacetate Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Bos taurus 5739
-

Organism

Organism UniProt Comment Textmining
Bos taurus
-
The classification is ambiguous because the stereochemistry of the reaction product is not exactly determined
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E)-octenoyl-CoA + H2O 36% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined Bos taurus ?
-
?
crotonyl-CoA + H2O best substrate. The classification is ambiguous because the stereochemistry is not exactly determined Bos taurus ?
-
?
decenoyl-CoA + H2O 17% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined Bos taurus ?
-
?
dodecenoyl-CoA + H2O 7% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined Bos taurus ?
-
?
hexadecenoyl-CoA + H2O 1% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined Bos taurus ?
-
?
hexenoyl-CoA + H2O 67% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined Bos taurus ?
-
?
tetradecenoyl-CoA + H2O 2% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined Bos taurus ?
-
?

Subunits

Subunits Comment Organism
hexamer
-
Bos taurus

Synonyms

Synonyms Comment Organism
crotonase the classification is ambiguous because the stereochemistry is not exactly determined Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5667
-
crotonyl-CoA pH 7.5, 25°C Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00024
-
hexenoyl-CoA pH 7.5, 25°C Bos taurus
0.00028
-
octenoyl-CoA pH 7.5, 25°C Bos taurus
0.0003
-
decenoyl-CoA pH 7.5, 25°C Bos taurus
0.0004
-
dodecenoyl-CoA pH 7.5, 25°C Bos taurus
0.00042
-
tetradecenoyl-CoA pH 7.5, 25°C Bos taurus
0.0005
-
hexadecenoyl-CoA pH 7.5, 25°C Bos taurus
0.0016
-
acetoacetyl-CoA pH 7.5, 25°C Bos taurus