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Literature summary for 4.2.1.17 extracted from

  • Waterson, R.M.; Castellino, F.J.; Hass, G.M.; Hill, R.L.
    Purification and characterization of cortonase from Clostridium acetobutylicum (1972), J. Biol. Chem., 247, 5266-5271.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
crotonyl-CoA
-
Clostridium acetobutylicum
additional information no inhibition by hexenoyl-CoA Clostridium acetobutylicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
crotonyl-CoA 25°C Clostridium acetobutylicum
0.13
-
hexenoyl-CoA 25°C Clostridium acetobutylicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
4 * 40000, SDS-PAGE Clostridium acetobutylicum
158000
-
equilibrium sedimentation analysis, gel filtration Clostridium acetobutylicum

Organism

Organism UniProt Comment Textmining
Clostridium acetobutylicum
-
The classification is ambiguous because the stereochemistry of the reaction product is not exactly determined
-

Purification (Commentary)

Purification (Comment) Organism
-
Clostridium acetobutylicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6155
-
-
Clostridium acetobutylicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
crotonyl-CoA + H2O the classification is ambiguous because the stereochemistry is not exactly determined Clostridium acetobutylicum ?
-
?
hexenoyl-CoA + H2O
-
Clostridium acetobutylicum ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 40000, SDS-PAGE Clostridium acetobutylicum

Synonyms

Synonyms Comment Organism
crotonase the classification is ambiguous because the stereochemistry of the reaction product is not exactly determined Clostridium acetobutylicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Clostridium acetobutylicum