Any feedback?
Literature summary for 4.2.1.169 extracted from
- Stereochemical conversion of C3-vinyl group to 1-hydroxyethyl group in bacteriochlorophyll c by the hydratases BchF and BchV adaptation of green sulfur bacteria to limited-light environments (2015), Mol. Microbiol., 98, 1184-1198 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene bchV, phylogenetic analysis, recombinant expression in Escherichia coli strain Bl21(DE3), quantitative RT-PCR expression analysis | Chlorobaculum tepidum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | bchV deletion mutant, mutant phenotype with altered pigment biosynthesis, detailed overview | Chlorobaculum tepidum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-vinyl bacteriochlorophyllide a + H2O | Chlorobaculum tepidum | - |
(31RS)-3-(1-hydroxyethyl) bacteriochlorophyllide a | - |
? |  |
| 3-vinyl bacteriochlorophyllide a + H2O | Chlorobaculum tepidum ATCC 49652 / DSM 12025 / NBRC 103806 / TLS | - |
(31RS)-3-(1-hydroxyethyl) bacteriochlorophyllide a | - |
? | |
| 3-vinyl bacteriochlorophyllide c + H2O | Chlorobaculum tepidum | low activity | (31S)-3-(1-hydroxyethyl) bacteriochlorophyllide c | - |
? | |
| 3-vinyl bacteriochlorophyllide c + H2O | Chlorobaculum tepidum ATCC 49652 / DSM 12025 / NBRC 103806 / TLS | low activity | (31S)-3-(1-hydroxyethyl) bacteriochlorophyllide c | - |
? | |
| 3-vinyl bacteriochlorophyllide d + H2O | Chlorobaculum tepidum | high activity, the enzyme converts [E,M], [E,E], [P,E], and [E,I] variants, the [E,E] and [P,E] variants are preferred substrates, stereochemistry, overview | (3RS)-3-(1-hydroxyethyl) bacteriochlorophyllide d | - |
? | |
| 3-vinyl-8,12-diethyl-bacteriochlorophyllide d + H2O | Chlorobaculum tepidum | - |
(31R)-(1-hydroxyethyl)-8,12-diethyl-bacteriochlorophyllide d | - |
? | |
| 3-vinyl-8,12-diethyl-bacteriochlorophyllide d + H2O | Chlorobaculum tepidum ATCC 49652 / DSM 12025 / NBRC 103806 / TLS | - |
(31R)-(1-hydroxyethyl)-8,12-diethyl-bacteriochlorophyllide d | - |
? | |
| 3-vinyl-8-ethyl-12-methyl-bacteriochlorophyllide d + H2O | Chlorobaculum tepidum | - |
(31R)-(1-hydroxyethyl)-8-ethyl-12-methyl-bacteriochlorophyllide d | - |
? | |
| 3-vinyl-8-ethyl-12-methyl-bacteriochlorophyllide d + H2O | Chlorobaculum tepidum ATCC 49652 / DSM 12025 / NBRC 103806 / TLS | - |
(31R)-(1-hydroxyethyl)-8-ethyl-12-methyl-bacteriochlorophyllide d | - |
? | |
| 3-vinyl-8-isobutyl-12-ethyl-bacteriochlorophyllide d + H2O | Chlorobaculum tepidum | - |
(31RS)3-(1-hydroxyethyl) bacteriochlorophyllide d | - |
? | |
| 3-vinyl-8-propyl-12-ethyl-bacteriochlorophyllide d + H2O | Chlorobaculum tepidum | - |
(3RS)3-(1-hydroxyethyl)-8-propyl-12-ethyl-bacteriochlorophyllide d | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlorobaculum tepidum | - |
- |
- |
| Chlorobaculum tepidum ATCC 49652 / DSM 12025 / NBRC 103806 / TLS | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-vinyl bacteriochlorophyllide a + H2O | - |
Chlorobaculum tepidum | (31RS)-3-(1-hydroxyethyl) bacteriochlorophyllide a | - |
? | |
| 3-vinyl bacteriochlorophyllide a + H2O | - |
Chlorobaculum tepidum ATCC 49652 / DSM 12025 / NBRC 103806 / TLS | (31RS)-3-(1-hydroxyethyl) bacteriochlorophyllide a | - |
? | |
| 3-vinyl bacteriochlorophyllide c + H2O | low activity | Chlorobaculum tepidum | (31S)-3-(1-hydroxyethyl) bacteriochlorophyllide c | - |
? | |
| 3-vinyl bacteriochlorophyllide c + H2O | low activity | Chlorobaculum tepidum ATCC 49652 / DSM 12025 / NBRC 103806 / TLS | (31S)-3-(1-hydroxyethyl) bacteriochlorophyllide c | - |
? | |
| 3-vinyl bacteriochlorophyllide d + H2O | high activity, the enzyme converts [E,M], [E,E], [P,E], and [E,I] variants, the [E,E] and [P,E] variants are preferred substrates, stereochemistry, overview | Chlorobaculum tepidum | (3RS)-3-(1-hydroxyethyl) bacteriochlorophyllide d | - |
? | |
| 3-vinyl-8,12-diethyl-bacteriochlorophyllide d + H2O | - |
Chlorobaculum tepidum | (31R)-(1-hydroxyethyl)-8,12-diethyl-bacteriochlorophyllide d | - |
? | |
| 3-vinyl-8,12-diethyl-bacteriochlorophyllide d + H2O | - |
Chlorobaculum tepidum ATCC 49652 / DSM 12025 / NBRC 103806 / TLS | (31R)-(1-hydroxyethyl)-8,12-diethyl-bacteriochlorophyllide d | - |
? | |
| 3-vinyl-8-ethyl-12-methyl-bacteriochlorophyllide d + H2O | - |
Chlorobaculum tepidum | (31R)-(1-hydroxyethyl)-8-ethyl-12-methyl-bacteriochlorophyllide d | - |
? | |
| 3-vinyl-8-ethyl-12-methyl-bacteriochlorophyllide d + H2O | - |
Chlorobaculum tepidum ATCC 49652 / DSM 12025 / NBRC 103806 / TLS | (31R)-(1-hydroxyethyl)-8-ethyl-12-methyl-bacteriochlorophyllide d | - |
? | |
| 3-vinyl-8-isobutyl-12-ethyl-bacteriochlorophyllide d + H2O | - |
Chlorobaculum tepidum | (31RS)3-(1-hydroxyethyl) bacteriochlorophyllide d | - |
? | |
| 3-vinyl-8-propyl-12-ethyl-bacteriochlorophyllide d + H2O | - |
Chlorobaculum tepidum | (3RS)3-(1-hydroxyethyl)-8-propyl-12-ethyl-bacteriochlorophyllide d | - |
? | |
| additional information | in vitro activity measurements with crude enzyme extract | Chlorobaculum tepidum | ? | - |
? | |
| additional information | in vitro activity measurements with crude enzyme extract | Chlorobaculum tepidum ATCC 49652 / DSM 12025 / NBRC 103806 / TLS | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BchV | - |
Chlorobaculum tepidum |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Chlorobaculum tepidum | transcriptional level of bchV is upregulated at lower light intensity, the Chlorobaculum tepidum adapts to low-light environments by control of the bchV transcription | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | pigment analyses of the bchF-inactivated mutant, which still has BchV as a sole hydratase, show higher ratios of S-epimeric bacteriochlorophyll c than the wild-type strain, while the bchV-mutant possessing only BchF shows a significant decrease of the S-epimers and accumulations of C3-vinyl BChl c species | Chlorobaculum tepidum |
| metabolism | proposed biosynthetic pathways of bacteriochlorophyllides a and c focused on Chlorobaculum tepidum BchF- and BchV-catalyzed reactions, overview | Chlorobaculum tepidum |
| physiological function | photosynthetic green sulfur bacteria inhabit anaerobic environments with very low-light conditions. Stereochemical conversion of C3-vinyl group to 1-hydroxyethyl group in bacteriochlorophyll c by the hydratases BchF and BchV (EC 4.2.1.169) for adaptation of green sulfur bacteria to limited-light environments. The pigment possess a hydroxy group at the C31 position that produces a chiral center with R- or S-stereochemistry and the C31-hydroxy group serves as a connecting moiety for the self-aggregation. Transcriptional level of bchV is upregulated at lower light intensity, the Chlorobaculum tepidum adapts to low-light environments by control of the bchV transcription | Chlorobaculum tepidum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
