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Literature summary for 4.2.1.167 extracted from

  • Thamer, W.; Cirpus, I.; Hans, M.; Pierik, A.J.; Selmer, T.; Bill, E.; Linder, D.; Buckel, W.
    A two [4Fe-4S]-cluster-containing ferredoxin as an alternative electron donor for 2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans (2003), Arch. Microbiol., 179, 197-204.
    View publication on PubMed
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Metals/Ions

Metals/Ions Comment Organism Structure
Molybdenum protein contains about 0.1 molybdenum (VI) per heterodimer. The enzyme has to be activated by the extremely oxygensensitive [4Fe-4S]1+/2+-cluster-containing homodimeric component A, which generates Mo(V) by an ATP/Mg2+-induced one-electron transfer Acidaminococcus fermentans

Organism

Organism UniProt Comment Textmining
Acidaminococcus fermentans P11569 and P11570 P11569 i.e. subunit alpha, P11570 i.e. subunit beta
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Acidaminococcus fermentans DSM 20731 P11569 and P11570 P11569 i.e. subunit alpha, P11570 i.e. subunit beta
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2-hydroxyglutaryl-CoA
-
 Acidaminococcus fermentans (E)-glutaconyl-CoA + H2O
-
 ?
(R)-2-hydroxyglutaryl-CoA
-
 Acidaminococcus fermentans DSM 20731 (E)-glutaconyl-CoA + H2O
-
 ?

Cofactor

Cofactor Comment Organism Structure
Ferredoxin alternative electron donor besides flavodoxin is a two [4Fe-4S]1+/2+-cluster-containing ferredoxin, with redox potentials of –405 mV and –340mV. The flavodoxin is the dominant electron donor protein under iron-limiting conditions. The concentration of ferredoxin increases stepwise from about 0.2 micromol/g at 7–13 microM Fe to 1.1 micromol/g at 17–45 microM Fe Acidaminococcus fermentans
flavodoxin dominant electron donor protein under iron-limiting conditions Acidaminococcus fermentans
riboflavin 5'-phosphate the actual dehydration is mediated by component D, which contains 1.0 [4Fe-4S]2+ cluster, 1.0 reduced riboflavin-5'-phosphate and about 0.1 molybdenum (VI) per heterodimer Acidaminococcus fermentans
[4Fe-4S]-center the actual dehydration is mediated by component D, which contains 1.0 [4Fe-4S]2+ cluster, 1.0 reduced riboflavin-5'-phosphate and about 0.1 molybdenum (VI) per heterodimer Acidaminococcus fermentans