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Literature summary for 4.2.1.167 extracted from

  • Schweiger, G.; Dutscho R.; Buckel, W.
    Purification of 2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans. An iron-sulfur protein (1987), Eur. J. Biochem., 169, 441-448.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Activator protein the enzyme must be activated by an activator protein. Once activated, it can catalyse many turnovers Acidaminococcus fermentans

Metals/Ions

Metals/Ions Comment Organism Structure
[4Fe-4S]2+ cluster
-
Acidaminococcus fermentans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
1 * 55000, 1 * 42000, SDS-PAGE Acidaminococcus fermentans
42000
-
2 * 55000 (alpha), + 2 * 42000 (beta), SDS-PAGE Acidaminococcus fermentans
55000
-
1 * 55000, 1 * 42000, SDS-PAGE Acidaminococcus fermentans
55000
-
2 * 55000 (alpha), + 2 * 42000 (beta), SDS-PAGE Acidaminococcus fermentans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-2-hydroxyglutaryl-CoA Acidaminococcus fermentans the enzyme is involved in the fermentation of L-glutamate (E)-glutaconyl-CoA + H2O
-
?
(R)-2-hydroxyglutaryl-CoA Acidaminococcus fermentans DSM 20731 the enzyme is involved in the fermentation of L-glutamate (E)-glutaconyl-CoA + H2O
-
?

Organism

Organism UniProt Comment Textmining
Acidaminococcus fermentans P11569 and P11570
-
-
Acidaminococcus fermentans P11569 and P11570 P11569: alpha-subunit, P11570: beta-subunit
-
Acidaminococcus fermentans DSM 20731 P11569 and P11570
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Acidaminococcus fermentans
chromatography on blue-Sepharose Acidaminococcus fermentans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0558
-
pH 7.0, 37°C Acidaminococcus fermentans

Storage Stability

Storage Stability Organism
4°C, stable for several months Acidaminococcus fermentans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2-hydroxyglutaryl-CoA
-
Acidaminococcus fermentans (E)-glutaconyl-CoA + H2O
-
?
(R)-2-hydroxyglutaryl-CoA
-
Acidaminococcus fermentans (E)-glutaconyl-CoA + H2O
-
r
(R)-2-hydroxyglutaryl-CoA the enzyme is involved in the fermentation of L-glutamate Acidaminococcus fermentans (E)-glutaconyl-CoA + H2O
-
?
(R)-2-hydroxyglutaryl-CoA
-
Acidaminococcus fermentans DSM 20731 (E)-glutaconyl-CoA + H2O
-
?
(R)-2-hydroxyglutaryl-CoA
-
Acidaminococcus fermentans DSM 20731 (E)-glutaconyl-CoA + H2O
-
r
(R)-2-hydroxyglutaryl-CoA the enzyme is involved in the fermentation of L-glutamate Acidaminococcus fermentans DSM 20731 (E)-glutaconyl-CoA + H2O
-
?

Subunits

Subunits Comment Organism
heterodimer 1 * 55000, 1 * 42000, SDS-PAGE Acidaminococcus fermentans
tetramer 2 * 55000 (alpha), + 2 * 42000 (beta), SDS-PAGE Acidaminococcus fermentans

Synonyms

Synonyms Comment Organism
hgdAB
-
Acidaminococcus fermentans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Acidaminococcus fermentans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Acidaminococcus fermentans

Cofactor

Cofactor Comment Organism Structure
4Fe-4S-center the enzyme contains [4F-4S] clusters Acidaminococcus fermentans

General Information

General Information Comment Organism
metabolism the enzyme is involved in the fermentation of L-glutamate Acidaminococcus fermentans