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Literature summary for 4.2.1.165 extracted from

  • Lange, C.; Kiesel, S.; Peters, S.; Virus, S.; Scheer, H.; Jahn, D.; Moser, J.
    Broadened substrate specificity of 3-hydroxyethyl bacteriochlorophyllide a dehydrogenase (BchC) indicates a new route for the biosynthesis of bacteriochlorophyll a (2015), J. Biol. Chem., 290, 19697-19709 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.165

Cloned(Commentary)

Cloned (Comment) Organism
gene bchF, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Chlorobaculum tepidum

Protein Variants

Protein Variants Comment Organism
additional information reconstitution of a bacteriochlorophyll formation pathway consisting of dark-operative protochlorophyllide oxidoreductase, BchF, and BchC, with chlorin 3-hydroxyethyl chlorophyllide as a natural substrate identified for BchC. Modification of the isocyclic E ring, omission of the central magnesium ion, zinc as an alternative metal ion, and a non-reduced B ring system are tolerated by BchC. According to this broadened in vitro activity, the chlorin 3-hydroxyethyl chlorophyllide a is identified as a natural substrate of BchC in a reconstituted pathway consisting of dark-operative protochlorophyllide oxidoreductase, BchF, and BchC. The established reaction sequence allows for an additional new branching point for the synthesis of bacteriochlorophyll a Chlorobaculum tepidum

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane integral transmembrane protein, the enzyme contains four putative membrane-spanning helices comprising the transmembrane segments Ile23-Tyr42, Thr56-Glu77, Phe94-Gln113, and His118-Gln140 Chlorobaculum tepidum 16020
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a 3-(1-hydroxyethyl) bacteriochlorophyllide a Chlorobaculum tepidum
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 a 3-vinyl bacteriochlorophyllide a + H2O
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 ?
a 3-(1-hydroxyethyl) bacteriochlorophyllide a Chlorobaculum tepidum ATCC 49652 / DSM 12025 / NBRC 103806 / TLS
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 a 3-vinyl bacteriochlorophyllide a + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Chlorobaculum tepidum Q8KBL0
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Chlorobaculum tepidum ATCC 49652 / DSM 12025 / NBRC 103806 / TLS Q8KBL0
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-

Purification (Commentary)

Purification (Comment) Organism
the recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) is efficiently isolated by detergent solubilization and ultracentrifugation from the host membrane fraction Chlorobaculum tepidum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a 3-(1-hydroxyethyl) bacteriochlorophyllide a
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 Chlorobaculum tepidum a 3-vinyl bacteriochlorophyllide a + H2O
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 ?
a 3-(1-hydroxyethyl) bacteriochlorophyllide a
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 Chlorobaculum tepidum ATCC 49652 / DSM 12025 / NBRC 103806 / TLS a 3-vinyl bacteriochlorophyllide a + H2O
-
 ?
additional information coupled enzymatic assays of BchF and BchC are initiated in an anoxic chamber, using either crude cellular BchF and BchC extracts or alternatively a BchF extract and the purified BchC protein, with dithionite as an artificial electron donor (NAD+/NADP+ in in vivo assays), dithiothreitol, ATP, and an ATP-regenerating system (creatine phosphate and creatine phosphokinase) for the analysis of BchC (EC 1.1.1.396) substrate specificity, overview Chlorobaculum tepidum ?
-
 ?
additional information coupled enzymatic assays of BchF and BchC are initiated in an anoxic chamber, using either crude cellular BchF and BchC extracts or alternatively a BchF extract and the purified BchC protein, with dithionite as an artificial electron donor (NAD+/NADP+ in in vivo assays), dithiothreitol, ATP, and an ATP-regenerating system (creatine phosphate and creatine phosphokinase) for the analysis of BchC (EC 1.1.1.396) substrate specificity, overview Chlorobaculum tepidum ATCC 49652 / DSM 12025 / NBRC 103806 / TLS ?
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 ?

Subunits

Subunits Comment Organism
? x * 20008, sequence calculation, x * 25000, recombinant His6-tagged enzyme, SDS-PAGE Chlorobaculum tepidum

Synonyms

Synonyms Comment Organism
3-vinyl bacteriochlorophyllide a hydratase
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 Chlorobaculum tepidum
bchF
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 Chlorobaculum tepidum

General Information

General Information Comment Organism
physiological function bacteriochlorophyll a requires formation of a 3-hydroxyethyl group on pyrrole ring A that is subsequently converted into a 3-acetyl group by 3-vinyl bacteriochlorophyllide a hydratase (BchF) followed by 3-hydroxyethyl bacteriochlorophyllide a dehydrogenase (BchC) Chlorobaculum tepidum