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Literature summary for 4.2.1.156 extracted from

  • Easton, N.M.; Aboushawareb, S.A.E.; Bearne, S.L.
    A continuous assay for L-talarate/galactarate dehydratase using circular dichroism (2018), Anal. Biochem., 544, 80-86 .
    View publication on PubMed
Search for more literature for 4.2.1.156

Cloned(Commentary)

Cloned (Comment) Organism
gene STM3697, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors Comment Organism Structure
additional information tartronate is a much weaker inhibitor of enzyme StTGD than of mandelate racemase Salmonella enterica subsp. enterica serovar Typhimurium
Tartronate competitive inhibition Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-talarate Salmonella enterica subsp. enterica serovar Typhimurium
-
 5-dehydro-4-deoxy-D-glucarate + H2O
-
 ?
L-talarate Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720
-
 5-dehydro-4-deoxy-D-glucarate + H2O
-
 ?
additional information Salmonella enterica subsp. enterica serovar Typhimurium the enzyme catalyzes the dehydration of either meso-galactarate or L-talarate via a common intermediate to form 5-keto-4-deoxy-D-glucarate (5-KDG) ?
-
 ?
additional information Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720 the enzyme catalyzes the dehydration of either meso-galactarate or L-talarate via a common intermediate to form 5-keto-4-deoxy-D-glucarate (5-KDG) ?
-
 ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium Q8ZL58
-
-
Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720 Q8ZL58
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21 DE3 by ultracentrifugation, nickel affinity chromatography, and dialysis Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-talarate
-
 Salmonella enterica subsp. enterica serovar Typhimurium 5-dehydro-4-deoxy-D-glucarate + H2O
-
 ?
L-talarate
-
 Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720 5-dehydro-4-deoxy-D-glucarate + H2O
-
 ?
additional information the enzyme catalyzes the dehydration of either meso-galactarate or L-talarate via a common intermediate to form 5-keto-4-deoxy-D-glucarate (5-KDG) Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
additional information the enzyme catalyzes the dehydration of either meso-galactarate or L-talarate via a common intermediate to form 5-keto-4-deoxy-D-glucarate (5-KDG) Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720 ?
-
 ?

Synonyms

Synonyms Comment Organism
L-talarate/galactarate dehydratase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
More cf. EC 4.2.1.42 Salmonella enterica subsp. enterica serovar Typhimurium
STM3697
-
 Salmonella enterica subsp. enterica serovar Typhimurium
StTGD
-
 Salmonella enterica subsp. enterica serovar Typhimurium
TGD
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

General Information

General Information Comment Organism
evolution L-talarate/galactarate dehydratase (TGD) is a member of the mandelate racemase (MR) subgroup of the enolase superfamily of enzymes. Members of the MR subgroup share with other members of the enolase superfamily a common bidomain structure (i.e., (beta/alpha)-7beta-barrel domain and an alpha+beta capping domain) and a common partial reaction (i.e., the metal-assisted, Broensted base-catalyzed abstraction of the alpha-proton from a carboxylate substrate to form an enol(ate) intermediate) Salmonella enterica subsp. enterica serovar Typhimurium