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Literature summary for 4.2.1.155 extracted from

  • Tan, D.; Crabb, W.M.; Whitman, W.B.; Tong, L.
    Crystal structure of DmdD, a crotonase superfamily enzyme that catalyzes the hydration and hydrolysis of methylthioacryloyl-CoA (2013), PLoS One, 8, e63870.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.155

Cloned(Commentary)

Cloned (Comment) Organism
the recombinant protein, with a C-terminal hexahistidine tag, was over-expressed in E. coli BL21 (DE3) Star cells Ruegeria pomeroyi

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of the wild-type enzyme and the E121A mutant enzyme are grown at 20°C by the hanging-drop vapor diffusion method. Crystal structure of the free enzyme at 1.5 A resolution, structures of the E121A mutant in complex with methylthioacryloyl-CoA and 3-methylmercaptopropionate-CoA at 1.8 A resolution Ruegeria pomeroyi

Protein Variants

Protein Variants Comment Organism
E121A crystal structures of the E121A mutant in complex with methylthioacryloyl-CoA and 3-methylmercaptopropionate-CoA at 1.8 A resolution Ruegeria pomeroyi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-(methylthio)acryloyl-CoA + 2 H2O Ruegeria pomeroyi the enzyme involved in the degradation of 3-(dimethylsulfonio)propanoate, an osmolyte produced by marine phytoplankton acetaldehyde + methanethiol + CoA + CO2
-
 ?
3-(methylthio)acryloyl-CoA + 2 H2O Ruegeria pomeroyi DSM 15171 the enzyme involved in the degradation of 3-(dimethylsulfonio)propanoate, an osmolyte produced by marine phytoplankton acetaldehyde + methanethiol + CoA + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Ruegeria pomeroyi Q5LLW6
-
-
Ruegeria pomeroyi DSM 15171 Q5LLW6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ruegeria pomeroyi

Reaction

Reaction Comment Organism Reaction ID
3-(methylsulfanyl)acryloyl-CoA + 2 H2O = acetaldehyde + methanethiol + CoA + CO2 overall reaction Ruegeria pomeroyi
a
3-(methylsulfanyl)acryloyl-CoA + H2O = 3-hydroxy-3-(methylsulfanyl)propanoyl-CoA (1a) Ruegeria pomeroyi
a
3-hydroxy-3-(methylsulfanyl)propanoyl-CoA = 3-oxopropanoyl-CoA + methanethiol (1b) Ruegeria pomeroyi
a
3-oxopropanoate = acetaldehyde + CO2 (1d) Ruegeria pomeroyi
a
3-oxopropanoyl-CoA + H2O = 3-oxopropanoate + CoA (1c) Ruegeria pomeroyi
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-(methylthio)acryloyl-CoA + 2 H2O the enzyme involved in the degradation of 3-(dimethylsulfonio)propanoate, an osmolyte produced by marine phytoplankton Ruegeria pomeroyi acetaldehyde + methanethiol + CoA + CO2
-
 ?
3-(methylthio)acryloyl-CoA + 2 H2O 3-(methylthio)acryloyl-CoA i.e. 3-(methylthio)prop-2-enoyl-CoA. 3-Methylmercaptopropionate-CoA cannot be hydrated and is only hydrolyzed slowly by the enzyme. Replacement of the sulfur atom in 3-(methylthio)acryloyl-CoA with a methylene group abolishes hydrolysis Ruegeria pomeroyi acetaldehyde + methanethiol + CoA + CO2
-
 ?
3-(methylthio)acryloyl-CoA + 2 H2O the enzyme involved in the degradation of 3-(dimethylsulfonio)propanoate, an osmolyte produced by marine phytoplankton Ruegeria pomeroyi DSM 15171 acetaldehyde + methanethiol + CoA + CO2
-
 ?
3-(methylthio)acryloyl-CoA + 2 H2O 3-(methylthio)acryloyl-CoA i.e. 3-(methylthio)prop-2-enoyl-CoA. 3-Methylmercaptopropionate-CoA cannot be hydrated and is only hydrolyzed slowly by the enzyme. Replacement of the sulfur atom in 3-(methylthio)acryloyl-CoA with a methylene group abolishes hydrolysis Ruegeria pomeroyi DSM 15171 acetaldehyde + methanethiol + CoA + CO2
-
 ?

Subunits

Subunits Comment Organism
hexamer composed of a dimer of trimers where the three monomers of each trimer are related by a crystallographic 3-fold axis Ruegeria pomeroyi

Synonyms

Synonyms Comment Organism
DmdD
-
 Ruegeria pomeroyi