Literature summary for 4.2.1.150 extracted from

Liu, S.; Yu, H.; Liu, Y.; Liu, X.; Zhang, Y.; Bu, C.; Yuan, S.; Chen, Z.; Xie, G.; Li, W.; Xu, B.; Yang, J.; He, L.; Jin, T.; Xiong, Y.; Sun, L.; Liu, X.; Han, C.; Cheng, Z.; Liang, J.; Shang, Y.
Chromodomain protein CDYL acts as a crotonyl-CoA hydratase to regulate histone crotonylation and spermatogenesis (2017), Mol. Cell, 67, 853-866.e5 .

Search for more literature for 4.2.1.150

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of Cdyl transgenic mice by microinjection of a Cdyl construct into the pro-nuclei of fertilized oocytes, derived from intercross of C57BL/6 3 CBA F1 mice	Mus musculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	steady-state kinetics of the CDYL-catalyzed hydratation reaction compared to mitochondrial metabolic enzyme enoyl-CoA hydratase (ECH, EC 4.2.1.17) as a positive control	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Homo sapiens	5739	-
mitochondrion	-	Mus musculus	5739	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
crotonyl-CoA + H2O	Homo sapiens	-	3-hydroxybutyryl-CoA	-	r
crotonyl-CoA + H2O	Mus musculus	-	3-hydroxybutyryl-CoA	-	r
crotonyl-CoA + H2O	Mus musculus C57BL/6	-	3-hydroxybutyryl-CoA	-	r

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9Y232	-	-
Mus musculus	Q9WTK2	-	-
Mus musculus C57BL/6	Q9WTK2	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
HeLa cell	-	Homo sapiens	-
spermatid	-	Homo sapiens	-
spermatid	-	Mus musculus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
crotonyl-CoA + H2O	-	Homo sapiens	3-hydroxybutyryl-CoA	-	r
crotonyl-CoA + H2O	-	Mus musculus	3-hydroxybutyryl-CoA	-	r
crotonyl-CoA + H2O	-	Mus musculus C57BL/6	3-hydroxybutyryl-CoA	-	r

Synonyms

Synonyms	Comment	Organism
CDYL	-	Homo sapiens
CDYL	-	Mus musculus
chromodomain Y-like protein	-	Homo sapiens
chromodomain Y-like protein	-	Mus musculus
crotonyl-CoA hydratase	-	Homo sapiens
crotonyl-CoA hydratase	-	Mus musculus
More	cf. EC 4.2.1.17	Homo sapiens
More	cf. EC 4.2.1.17	Mus musculus

General Information

General Information	Comment	Organism
evolution	the C-terminal CoAP domain of the CDY family proteins including CDYL has a three-dimensional structure closely resembling enoyl-CoA hydratase, which catalyzes the hydratation of 2-trans-enoyl-CoA into beta-hydroxyacyl-CoA in mitochondria during beta-oxidation of fatty acids	Homo sapiens
evolution	the C-terminal CoAP domain of the CDY family proteins including CDYL has a three-dimensional structure closely resembling enoyl-CoA hydratase, which catalyzes the hydratation of 2-trans-enoyl-CoA into beta-hydroxyacyl-CoA in mitochondria during beta-oxidation of fatty acids	Mus musculus
malfunction	Cdyl knockout mice are embryonically lethal or died shortly after birth with significantly reduced levels of histone Kcr in elongating spermatids from Cdyl transgenic mice compared to that from wild-type mice, although the level of histone acetylation is comparable	Mus musculus
malfunction	the levels of total histone Kcr and H2BK12cr on the promoter of known CDYL target genes BDNF, NEUROD1, SCG10, and MYT1 increase significantly in CDYL-KO HeLa cells, whereas the regional level of H3K27me3 in these cells decreases, expression patterns, overview	Homo sapiens
additional information	both the chromodomain and CoAP domain of CDYL are required for its negative regulation of histone Kcr	Homo sapiens
additional information	both the chromodomain and CoAP domain of CDYL are required for its negative regulation of histone Kcr	Mus musculus
physiological function	the chromodomain Y-like protein CDYL acts as a crotonyl-CoA hydratase to negatively regulate histone crotonylation. The chromodomain Y-like transcription corepressor CDYL negatively regulates histone Kcr by acting as a crotonyl-CoA hydratase to convert crotonyl-CoA to beta-hydroxybutyryl-CoA. This activity is intrinsically linked to the transcription repression function of CDYL and is implemented in reactivation of sex chromosome-linked genes and histone replacement during spermatogenesis. The negative regulation of histone Kcr by CDYL is intrinsically linked to its transcription repression activity and functionally implemented in the reactivation of sex chromosome-linked genes in round spermatids and genome-wide histone replacement in elongating spermatids	Mus musculus
physiological function	the chromodomain Y-like protein CDYL acts as a crotonyl-CoA hydratase to negatively regulate histone crotonylation. The chromodomain Y-like transcription corepressor CDYL negatively regulates histone Kcr by acting as a crotonyl-CoA hydratase to convert crotonyl-CoA to beta-hydroxybutyryl-CoA. This activity is intrinsically linked to the transcription repression function of CDYL and is implemented in reactivation of sex chromosome-linked genes and histone replacement during spermatogenesis. The negative regulation of histone Kcr by CDYL is intrinsically linked to its transcription repression activity and functionally implemented in the reactivation of sex chromosome-linked genes in round spermatids and genome-wide histone replacement in elongating spermatids. Cdyl regulates the expression of sex chromosome-linked escaped genes in postmeiotic spermatogenic cells by mainly influencing histone Kcr on the gene promoters. The enzyme is important in the physiology of male reproduction and the mechanism of the spermatogenic failure in AZFc (azoospermia factor c)-deleted infertile men	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)