Inhibitors | Comment | Organism | Structure |
---|---|---|---|
crotonyl-CoA | the enzyme is markedly sensitive to inhibition by crotonyl-CoA at concentrations greater than about 0.075 mM | Clostridium acetobutylicum | |
additional information | not inhibited by hexenoyl-CoA | Clostridium acetobutylicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
crotonyl-CoA | at pH 8.0 and 25°C | Clostridium acetobutylicum | |
0.13 | - |
hexenoyl-CoA | at pH 8.0 and 25°C | Clostridium acetobutylicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
4 * 40000, SDS-PAGE | Clostridium acetobutylicum |
158000 | - |
sedimentation equilibrium analysis and gel filtration | Clostridium acetobutylicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
crotonyl-CoA + H2O | Clostridium acetobutylicum | - |
3-hydroxybutyryl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium acetobutylicum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
acetone precipitation, Sephadex G-200 gel filtration, and DEAE-Sephadex column chromatography | Clostridium acetobutylicum |
Storage Stability | Organism |
---|---|
4 or -20°C, Tris-HCl, pH 8.0, rapid loss of activity | Clostridium acetobutylicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
crotonyl-CoA + H2O | - |
Clostridium acetobutylicum | 3-hydroxybutyryl-CoA | - |
? | |
hexenoyl-CoA + H2O | - |
Clostridium acetobutylicum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 40000, SDS-PAGE | Clostridium acetobutylicum |
Synonyms | Comment | Organism |
---|---|---|
crotonase | - |
Clostridium acetobutylicum |
L-3-hydroxyacyl-CoA hydro-lyase | - |
Clostridium acetobutylicum |
More | cf. EC 4.2.1.17 | Clostridium acetobutylicum |