Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43600 | - |
2 * 43600, SDS-PAGE | Proteus sp. |
84200 | - |
gel filtration | Proteus sp. |
91100 | - |
calculated from amino acid sequence | Proteus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-carnitinyl-CoA | Proteus sp. | - |
(E)-4-(trimethylammonio)but-2-enoyl-CoA + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Proteus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, phenyl-Sepharose column chromatography, Resource Q column chromatography, and Superose 12HR gel filtration | Proteus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3 | - |
cell free extract, in 10 mM sodium phosphate buffer (pH 7.8), at 37°C | Proteus sp. |
450 | - |
after 150fold purification, in 10 mM sodium phosphate buffer (pH 7.8), at 37°C | Proteus sp. |
Storage Stability | Organism |
---|---|
0 or 20°C, purified enzyme in 50 mM sodium phosphate buffer containing 150 mM NaCl, 48 h, complete loss of activity | Proteus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-carnitinyl-CoA | - |
Proteus sp. | (E)-4-(trimethylammonio)but-2-enoyl-CoA + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 43600, SDS-PAGE | Proteus sp. |
Synonyms | Comment | Organism |
---|---|---|
CaiD | - |
Proteus sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Proteus sp. | calculated from amino acid sequence | - |
5 |