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Literature summary for 4.2.1.148 extracted from

  • Zarzycki, J.; Schlichting, A.; Strychalsky, N.; Muller, M.; Alber, B.E.; Fuchs, G.
    Mesaconyl-coenzyme A hydratase, a new enzyme of two central carbon metabolic pathways in bacteria (2008), J. Bacteriol., 190, 1366-1374.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.148

Cloned(Commentary)

Cloned (Comment) Organism
heterologously expressed in Escherichia coli Cereibacter sphaeroides
heterologously expressed in Escherichia coli Chloroflexus aurantiacus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 2 * 40000, SDS-PAGE Cereibacter sphaeroides
40000
-
 2 * 40000, SDS-PAGE Chloroflexus aurantiacus
80000
-
 gel filtration Cereibacter sphaeroides
80000
-
 gel filtration Chloroflexus aurantiacus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2R,3S)-2-methylmalyl-CoA Cereibacter sphaeroides the enzyme is part of the 3-hydroxypropanoate cycle for carbon assimilation 2-methylfumaryl-CoA + H2O
-
 r
(2R,3S)-2-methylmalyl-CoA Chloroflexus aurantiacus the enzyme is part of the 3-hydroxypropanoate cycle for carbon assimilation 2-methylfumaryl-CoA + H2O
-
 r

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-
Chloroflexus aurantiacus A9WC34
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cereibacter sphaeroides
-
 Chloroflexus aurantiacus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1300
-
 55°C, pH not specified in the publication, dehydration of erythro-beta-methylmalyl-CoA to mesaconyl-CoA Chloroflexus aurantiacus
1400
-
 30°C, pH not specified in the publication, dehydration of erythro-beta-methylmalyl-CoA to mesaconyl-CoA Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-2-methylmalyl-CoA the enzyme is part of the 3-hydroxypropanoate cycle for carbon assimilation Cereibacter sphaeroides 2-methylfumaryl-CoA + H2O
-
 r
(2R,3S)-2-methylmalyl-CoA the enzyme is part of the 3-hydroxypropanoate cycle for carbon assimilation Chloroflexus aurantiacus 2-methylfumaryl-CoA + H2O
-
 r
(2R,3S)-2-methylmalyl-CoA (2R,3S)-2-methylmalyl-CoA i.e. L-erythro-beta-methylmalyl-CoA = (2R,3S)-2-methyl-3-carboxy-3-hydroxypropanoyl-CoA Cereibacter sphaeroides 2-methylfumaryl-CoA + H2O 2-methylfumaryl-CoA i.e. (E)-3-carboxy-2-methylprop-2-enoyl-CoA r
(2R,3S)-2-methylmalyl-CoA (2R,3S)-2-methylmalyl-CoA i.e. L-erythro-beta-methylmalyl-CoA = (2R,3S)-2-methyl-3-carboxy-3-hydroxypropanoyl-CoA Chloroflexus aurantiacus 2-methylfumaryl-CoA + H2O 2-methylfumaryl-CoA i.e. (E)-3-carboxy-2-methylprop-2-enoyl-CoA r

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE Cereibacter sphaeroides
dimer 2 * 40000, SDS-PAGE Chloroflexus aurantiacus
dimer 2 *37000, calculated from sequence Cereibacter sphaeroides
dimer 2 *38000, calculated from sequence Chloroflexus aurantiacus

Synonyms

Synonyms Comment Organism
erythro-beta-methylmalonyl-CoA hydrolyase
-
 Cereibacter sphaeroides
erythro-beta-methylmalonyl-CoA hydrolyase
-
 Chloroflexus aurantiacus
MCD
-
 Cereibacter sphaeroides
MCD
-
 Chloroflexus aurantiacus
mesaconyl-C1-CoA hydratase
-
 Cereibacter sphaeroides
mesaconyl-C1-CoA hydratase
-
 Chloroflexus aurantiacus
mesaconyl-CoA hydratase
-
 Cereibacter sphaeroides
mesaconyl-CoA hydratase
-
 Chloroflexus aurantiacus
mesaconyl-coenzyme A hydratase ambiguous Cereibacter sphaeroides
mesaconyl-coenzyme A hydratase ambiguous Chloroflexus aurantiacus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1700
-
 (2R,3S)-2-methylmalyl-CoA 55°C, pH not specified in the publication, dehydration of erythro-beta-methylmalyl-CoA to mesaconyl-CoA Chloroflexus aurantiacus
1900
-
 (2R,3S)-2-methylmalyl-CoA 30°C, pH not specified in the publication, dehydration of erythro-beta-methylmalyl-CoA to mesaconyl-CoA Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Cereibacter sphaeroides
7.5
-
-
 Chloroflexus aurantiacus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 half-maximal activity at pH 6.5 and pH 8.5 Cereibacter sphaeroides
6.5 8.5 half-maximal activity at pH 6.5 and pH 8.5 Chloroflexus aurantiacus

General Information

General Information Comment Organism
physiological function the enzyme is part of the 3-hydroxypropanoate cycle for carbon assimilation Cereibacter sphaeroides
physiological function the enzyme is part of the 3-hydroxypropanoate cycle for carbon assimilation Chloroflexus aurantiacus