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Literature summary for 4.2.1.147 extracted from
- Novel formaldehyde-activating enzyme in Methylobacterium extorquens AM1 required for growth on methanol (2000), J. Bacteriol., 182, 6645-6650.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
formaldehyde | 30°C, pH 8.0 | Methylorubrum extorquens |  |
| 1 | - |
5,6,7,8-tetrahydromethanopterin | 30°C, pH 8.0 | Methylorubrum extorquens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | one of the major proteins in the cytoplasm | Methylorubrum extorquens | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | stimulates activity, Ka: 4 mM | Methylorubrum extorquens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18000 | - |
3 * 18000, SDS-PAGE | Methylorubrum extorquens |
| 60000 | - |
gel filtration | Methylorubrum extorquens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6,7,8-tetrahydromethanopterin + formaldehyde | Methylorubrum extorquens | - |
5,10-methylenetetrahydromethanopterin + H2O | - |
? | |
| 5,6,7,8-tetrahydromethanopterin + formaldehyde | Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | - |
5,10-methylenetetrahydromethanopterin + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylorubrum extorquens | Q9FA38 | - |
- |
| Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | Q9FA38 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methylorubrum extorquens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20.2 | - |
30°C, pH 8.0 | Methylorubrum extorquens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6,7,8-tetrahydromethanopterin + formaldehyde | - |
Methylorubrum extorquens | 5,10-methylenetetrahydromethanopterin + H2O | - |
? | |
| 5,6,7,8-tetrahydromethanopterin + formaldehyde | the reaction also proceeds spontaneously, but at a lower rate than that of the enzyme-catalyzed reaction | Methylorubrum extorquens | 5,10-methylenetetrahydromethanopterin + H2O | - |
? | |
| 5,6,7,8-tetrahydromethanopterin + formaldehyde | - |
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | 5,10-methylenetetrahydromethanopterin + H2O | - |
? | |
| 5,6,7,8-tetrahydromethanopterin + formaldehyde | the reaction also proceeds spontaneously, but at a lower rate than that of the enzyme-catalyzed reaction | Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | 5,10-methylenetetrahydromethanopterin + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotrimer | 3 * 18000, SDS-PAGE | Methylorubrum extorquens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| formaldehyde-activating enzyme | - |
Methylorubrum extorquens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Methylorubrum extorquens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Methylorubrum extorquens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | mutants of Methylobacterium extorquens AM1 defective in Fae are able to grow on succinate but not on methanol and are much more sensitive toward methanol and formaldehyde | Methylorubrum extorquens |
| physiological function | the enzyme is required for growth on methanol | Methylorubrum extorquens |
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