Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-deoxy-D-arabinoheptulosonic acid 7-phosphate | 1 mM, 40-50% activation | Amycolatopsis mediterranei |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Amycolatopsis mediterranei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.164 | - |
5-amino-5-deoxy-3-dehydroshikimate | pH 7.5, 28°C | Amycolatopsis mediterranei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
2 * 39000, SDS-PAGE, 2 * 46101, calculated | Amycolatopsis mediterranei |
46101 | - |
2 * 39000, SDS-PAGE, 2 * 46101, calculated | Amycolatopsis mediterranei |
74000 | - |
gel filtration | Amycolatopsis mediterranei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amycolatopsis mediterranei | O52552 | - |
- |
Purification (Comment) | Organism |
---|---|
both native and recombinant protein | Amycolatopsis mediterranei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
72 | - |
pH 7.5, 28°C, native enzyme | Amycolatopsis mediterranei |
195 | - |
pH 7.5, 28°C, recombinant enzyme | Amycolatopsis mediterranei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-amino-5-deoxy-3-dehydroshikimate | - |
Amycolatopsis mediterranei | 3-amino-5-hydroxybenzoate + H2O | - |
? | |
additional information | no substrates: 5-deoxy-5-amino-3-dehydroquinic acid, 5-deoxy-5-aminoshikimic acid, quinic acid, 3-dehydroquinic acid, or 3-dehydroshikimic acid | Amycolatopsis mediterranei | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 39000, SDS-PAGE, 2 * 46101, calculated | Amycolatopsis mediterranei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
33 | - |
- |
Amycolatopsis mediterranei |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | 50 | more than 84% of maximum activity within | Amycolatopsis mediterranei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Amycolatopsis mediterranei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | enzyme is a dimer containing one molecule of pyridoxal phosphate per subunit. The enzyme-bound pyridoxal phosphate forms a Schiffs base with the amino group of 5-deoxy-5-amino-3-dehydroshikimic acid and catalyzes both an alpha,beta-dehydration and a stereospecific 1,4-enolization of the substrate | Amycolatopsis mediterranei |