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Literature summary for 4.2.1.141 extracted from

  • Brouns, S.J.; Walther, J.; Snijders, A.P.; van de Werken, H.J.; Willemen, H.L.; Worm, P.; de Vos, M.G.; Andersson, A.; Lundgren, M.; Mazon, H.F.; van den Heuvel, R.H.; Nilsson, P.; Salmon, L.; de Vos, W.M.; Wright, P.C.; Bernander, R.; van der Oost, J.
    Identification of the missing links in prokaryotic pentose oxidation pathways: evidence for enzyme recruitment (2006), J. Biol. Chem., 281, 27378-27388.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information multiple sequence alignment analysis of the enzyme indicates the presence of a metal binding site consisting of Glu143, Glu145, and Asp164, which may implicate a metal dependent activity Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33143
-
4 * 33143, calculated from sequence Saccharolobus solfataricus
132900
-
mass spectrometry under reducing conditions Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-dehydro-3-deoxy-D-arabinonate Saccharolobus solfataricus the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway 2,5-dioxopentanoate + H2O
-
?
2-dehydro-3-deoxy-D-arabinonate Saccharolobus solfataricus P2 the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway 2,5-dioxopentanoate + H2O
-
?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97UA0
-
-
Saccharolobus solfataricus P2 Q97UA0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-3-deoxy-D-arabinonate the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway Saccharolobus solfataricus 2,5-dioxopentanoate + H2O
-
?
2-dehydro-3-deoxy-D-arabinonate the catalytic activity of the protein is investigated by performing indirect enzyme assays using arabinonate dehydratase with D-arabinonate as a substrate. A 50% decrease in the yield of 2-dehydro-3-deoxy-D-arabinonate is observed when both enzymes are co-incubated in the presence of Mg2+. Given the fact that D-arabinonate is converted to 2-oxoglutarate in D-arabinose cell-free extract, this enzyme is anticipated to be responsible for the dehydration of 2-dehydro-3-deoxy-D-arabinonate to the aldehyde 2,5-dioxopentanoate. Due to the unavailability of 2-dehydro-3-deoxy-D-arabinonate, it is not possible to show this in a direct enzyme assay. 2-Oxoglutarate is produced in an indirect assay using arabinonate dehydratase, the putative 2-dehydro-3-deoxy-D-arabinonate dehydratase and the predicted aldehyde dehydrogenase Saccharolobus solfataricus 2,5-dioxopentanoate + H2O
-
?
2-dehydro-3-deoxy-D-arabinonate the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway Saccharolobus solfataricus P2 2,5-dioxopentanoate + H2O
-
?
2-dehydro-3-deoxy-D-arabinonate the catalytic activity of the protein is investigated by performing indirect enzyme assays using arabinonate dehydratase with D-arabinonate as a substrate. A 50% decrease in the yield of 2-dehydro-3-deoxy-D-arabinonate is observed when both enzymes are co-incubated in the presence of Mg2+. Given the fact that D-arabinonate is converted to 2-oxoglutarate in D-arabinose cell-free extract, this enzyme is anticipated to be responsible for the dehydration of 2-dehydro-3-deoxy-D-arabinonate to the aldehyde 2,5-dioxopentanoate. Due to the unavailability of 2-dehydro-3-deoxy-D-arabinonate, it is not possible to show this in a direct enzyme assay. 2-Oxoglutarate is produced in an indirect assay using arabinonate dehydratase, the putative 2-dehydro-3-deoxy-D-arabinonate dehydratase and the predicted aldehyde dehydrogenase Saccharolobus solfataricus P2 2,5-dioxopentanoate + H2O
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 33143, calculated from sequence Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
D-KDA dehydratase
-
Saccharolobus solfataricus
KdaD
-
Saccharolobus solfataricus
Sso3118
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
assay at Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Saccharolobus solfataricus

pI Value

Organism Comment pI Value Maximum pI Value
Saccharolobus solfataricus calculated from sequence
-
5.9