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Literature summary for 4.2.1.140 extracted from
- The nonphosphorylative Entner-Doudoroff pathway in the thermoacidophilic euryarchaeon Picrophilus torridus involves a novel 2-keto-3-deoxygluconate-specific aldolase (2010), J. Bacteriol., 192, 964-974.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
D-galactonate | pH 6.2, 60°C | Picrophilus torridus |  |
| 2.5 | - |
D-gluconate | pH 6.2, 60°C | Picrophilus torridus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, Km: 4.2 mM | Picrophilus torridus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
8 * 44000, SDS-PAGE | Picrophilus torridus |
| 340000 | - |
gel filtration | Picrophilus torridus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactonate | Picrophilus torridus | - |
2-dehydro-3-deoxy-D-galactonate + H2O | - |
? | |
| D-gluconate | Picrophilus torridus | - |
2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Picrophilus torridus | Q6L1T2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Picrophilus torridus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.1 | - |
substrate: D-gluconate, pH 6.2, 60°C | Picrophilus torridus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactonate | - |
Picrophilus torridus | 2-dehydro-3-deoxy-D-galactonate + H2O | - |
? | |
| D-galactonate | the ratio of catalytic efficiency for gluconate and galactonate utilization is about 10 to 1 | Picrophilus torridus | 2-dehydro-3-deoxy-D-galactonate + H2O | - |
? | |
| D-gluconate | - |
Picrophilus torridus | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
| D-gluconate | the ratio of catalytic efficiency for gluconate and galactonate utilization is about 10 to 1 | Picrophilus torridus | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
| additional information | no activity with xylonate | Picrophilus torridus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homooctamer | 8 * 44000, SDS-PAGE | Picrophilus torridus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Pto0485 | - |
Picrophilus torridus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
assay at | Picrophilus torridus |
| 70 | - |
- |
Picrophilus torridus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
2 h, stable | Picrophilus torridus |
| 70 | - |
half-life: 15 min | Picrophilus torridus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Picrophilus torridus |
| 6.2 | - |
assay at | Picrophilus torridus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8.1 | pH 4.5: 50% of maximal activity, pH 8.1: about 50% of maximal activity | Picrophilus torridus |
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