Literature summary for 4.2.1.136 extracted from

Marbaix, A.; No�l, G.; Detroux, A.; Vertommen, D.; Van Schaftingen, E.; Linster, C.
Extremely conserved ATP- or ADP-dependent enzymatic system for nicotinamide nucleotide (2011), J. Biol. Chem., 286, 41246-41252.

Search for more literature for 4.2.1.136

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli as a His-tagged fusion protein	Mus musculus
expressed in Escherichia coli as a His-tagged fusion protein	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Mus musculus	-	-	-
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate and further purification using three successive chromatographic steps on Blue Trisacryl, Source 30Q and superdex 200	Mus musculus
ammonium sulfate and further purification using three successive chromatographic steps on Blue Trisacryl, Source 30Q and superdex 200	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + NADHX	YjeF is bi-functional and ADP-dependent. YjeF not only dehydrates (S)-NAD(P)HX, but also interconverts (R)- and (S)-NAD(P)HX. For YjeF, (S)-NADPHX is a better substrate for this enzyme than (S)-NADHX, as indicated by a 2fold higher catalytic efficiency with (S)-NADPHX	Escherichia coli	AMP + phosphate + NADH	-	?
ATP + NADHX	the resulting mixture contains, in addition to (S)-NAD(P)HX and (R)-NAD(P)HX (in a 60:40 ratio), non-reacted NAD(P)H and cyclic forms derived from NAD(P)HX. (S)-NADPHX is a better substrate for this enzyme than (S)-NADHX, as indicated by a 2fold higher catalytic efficiency with (S)-NADPHX	Saccharomyces cerevisiae	ADP + phosphate + NADH	-	?
ATP + NADHX	the resulting mixture contains, in addition to (S)-NAD(P)HX and (R)-NAD(P)HX (in a 60:40 ratio), non-reacted NAD(P)H and cyclic forms derived from NAD(P)HX. For Carkd, a 4fold higher Vmax value is found when (S)-NADPHX is used as a substrate instead of (S)-NADHX	Mus musculus	ADP + phosphate + NADH	-	?

Synonyms

Synonyms	Comment	Organism
Carkd	-	Mus musculus
NADHX dehydratase	-	Mus musculus
NADHX dehydratase	-	Saccharomyces cerevisiae
YjeF	-	Escherichia coli
YKL151C	-	Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Mus musculus
30	-	assay at	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.1	-	assay at	Mus musculus
7.1	-	assay at	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)