Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.133 extracted from

  • Pateraki, I.; Andersen-Ranberg, J.; Hamberger, B.; Heskes, A.M.; Martens, H.J.; Zerbe, P.; Bach, S.S.; Moller, B.L.; Bohlmann, J.; Hamberger, B.
    Manoyl oxide (13R), the biosynthetic precursor of forskolin, is synthesized in specialized root cork cells in Coleus forskohlii (2014), Plant Physiol., 164, 1222-1236 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.133

Cloned(Commentary)

Cloned (Comment) Organism
gene TPS2, phylogenetic analysis Plectranthus barbatus

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information manoyl oxide is detected in root cork and, more specifically, in oil bodies isolated from this tissue, as well as in the root cortex and stele, stem, and flowers Plectranthus barbatus
-
-
oil body localization of forskolin and (13R)-manoyl oxide in root cork oil bodies, overview. Small amounts are located also in a small number of oil bodies in stem cork tissue Plectranthus barbatus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate + H2O Plectranthus barbatus
-
 (13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate
-
 r

Organism

Organism UniProt Comment Textmining
Plectranthus barbatus X4ZWN5 i.e. Coleus barbatus or Plectranthus barbatus
-

Reaction

Reaction Comment Organism Reaction ID
(13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate = geranylgeranyl diphosphate + H2O the class II diTPS reaction, which forms the bicyclic decalin core of labdanetype diterpenoids, is terminated either by deprotonation or by water capture of the diphosphate carbocation Plectranthus barbatus
a

Source Tissue

Source Tissue Comment Organism Textmining
cork a specialized cell type containing characteristic structures with histochemical properties consistent with oil bodies Plectranthus barbatus
-
root cork cell Plectranthus barbatus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate + H2O
-
 Plectranthus barbatus (13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate
-
 r
additional information compounds GC-MS analysis Plectranthus barbatus ?
-
 ?

Synonyms

Synonyms Comment Organism
CfTPS2
-
 Plectranthus barbatus
TPS2
-
 Plectranthus barbatus

General Information

General Information Comment Organism
evolution the enzyme is a class II diterpene synthase (diTPS), expression profiling and phylogenetic analysis of the CfTPS family. Coleus forskohlii diTPSs constitute a small gene family. Schematic overview of the biosynthetic routes from GGPP to specialized and general diterpenoids of the abietane, labdane, and ent-kaurene class Plectranthus barbatus
metabolism enzyme CfTPS2 synthesizes the intermediate copal-8-ol diphosphate,which in combination with CfTPS3 results in the stereospecific formation of (13R) manoyl oxide, while the combination of CfTPS1 an CfTPS3 or CfTPS4 leads to formation of miltiradiene, precursor of abietane diterpenoids in Coleus forskohlii, in combination with TPS14, (13R) and (13S) manoyl oxides are fomed Plectranthus barbatus
additional information analysis of diterpenoids in oil bodies from Coleus forskohlii, overview Plectranthus barbatus
physiological function involvement of CfTPS2 in the formation of (13R) manoyl oxide en route to forskolin in root cork cells Plectranthus barbatus