Cloned (Comment) | Organism |
---|---|
gene crtC, sequence comparisons and rooted phylogenetic tree, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli TOP10 cells and strain BL21(DE3). While the removal of the N-terminus result in an increased expression level, all point mutations negatively influences the expression of the protein | Rubrivivax gelatinosus |
gene crtC, sequence comparisons and rooted phylogenetic tree, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli TOP10 cells and strain BL21(DE3). While the removal of the N-terminus result in an increased expression level, all point mutations negatively influences the expression of the protein | Thiocapsa roseopersicina |
Protein Variants | Comment | Organism |
---|---|---|
D266A | site-directed mutagenesis, inactive mutant | Thiocapsa roseopersicina |
D268A | site-directed mutagenesis, inactive mutant | Rubrivivax gelatinosus |
H237A | site-directed mutagenesis, inactive mutant | Thiocapsa roseopersicina |
H239A | site-directed mutagenesis, inactive mutant | Rubrivivax gelatinosus |
H262A | site-directed mutagenesis, the mutant shows highly increased activity compared to the wild-type enzyme | Thiocapsa roseopersicina |
H264A | site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme | Rubrivivax gelatinosus |
additional information | expression levels of wild-type and mutant enzymes from Escherichia coli strain BL21(DE3), overview. While the removal of the N-terminus result in an increased expression level, all point mutations negatively influences the expression of the protein. The N-terminally trubcated mutant also shows increased activity compared to the wild-type enzyme | Thiocapsa roseopersicina |
additional information | expression levels of wild-type and mutant enzymes from Escherichia coli strain BL21(DE3), overview. While the removal of the N-terminus result in an increased expression level, all point mutations negatively influences the expression of the protein. The N-terminally truncated mutant also shows increased activity compared to the wild-type enzyme | Rubrivivax gelatinosus |
S58Q | site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme | Thiocapsa roseopersicina |
S58V | site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme | Thiocapsa roseopersicina |
W239A | site-directed mutagenesis, inactive mutant | Thiocapsa roseopersicina |
W241A | site-directed mutagenesis, inactive mutant | Rubrivivax gelatinosus |
Y266A | site-directed mutagenesis, inactive mutant | Rubrivivax gelatinosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 lycopene + H2O | Rubrivivax gelatinosus | cofactor-independent catalysis | 1-hydroxylycopene + 1,1'-dihydroxylycopene | - |
? | |
2 lycopene + H2O | Thiocapsa roseopersicina | cofactor-independent catalysis | 1-hydroxylycopene + 1,1'-dihydroxylycopene | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rubrivivax gelatinosus | P95619 | - |
- |
Thiocapsa roseopersicina | Q7X3G5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Rubrivivax gelatinosus |
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Thiocapsa roseopersicina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 lycopene + H2O | cofactor-independent catalysis | Rubrivivax gelatinosus | 1-hydroxylycopene + 1,1'-dihydroxylycopene | - |
? | |
2 lycopene + H2O | cofactor-independent catalysis | Thiocapsa roseopersicina | 1-hydroxylycopene + 1,1'-dihydroxylycopene | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 44000, recombinant His-tagged enzyme, SDS-PAGE | Rubrivivax gelatinosus |
? | x * 44000, recombinant His-tagged enzyme, SDS-PAGE | Thiocapsa roseopersicina |
Synonyms | Comment | Organism |
---|---|---|
CrtC | - |
Rubrivivax gelatinosus |
CrtC | - |
Thiocapsa roseopersicina |
RgCrtC | - |
Rubrivivax gelatinosus |
TrCrtC | - |
Thiocapsa roseopersicina |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rubrivivax gelatinosus |
25 | - |
assay at | Thiocapsa roseopersicina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Rubrivivax gelatinosus |
8 | - |
assay at | Thiocapsa roseopersicina |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the PF07143 family | Rubrivivax gelatinosus |
evolution | the enzyme belongs to the PF07143 family | Thiocapsa roseopersicina |