Cloned (Comment) | Organism |
---|---|
DJ-1 gene is subcloned from pLenti6-DJ-1-V5-WT plasmid into the pRetroX-Tight-Puro Advanced vector. The confirmed clone is transfected into HEK-293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
L166P | the Km-value for methylglyoxal of the mutant enzyme is about 2fold higher compared to the wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
900 | - |
methylglyoxal | 30°C, pH not specified in the publication, wild-type enzyme | Homo sapiens | |
1900 | - |
methylglyoxal | 30°C, pH not specified in the publication, mutant enzyme L166P | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylglyoxal + H2O | Homo sapiens | - |
(R)-lactate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q99497 | cf. EC 3.5.1.124 | - |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylglyoxal + H2O | - |
Homo sapiens | (R)-lactate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DJ-1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |