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Literature summary for 4.2.1.130 extracted from

  • Sharma, N.; Rao, S.P.; Kalivendi, S.V.
    The deglycase activity of DJ-1 mitigates alpha-synuclein glycation and aggregation in dopaminergic cells Role of oxidative stress mediated downregulation of DJ-1 in Parkinsons disease (2019), Free Radic. Biol. Med., 135, 28-37 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DJ-1 gene is subcloned from pLenti6-DJ-1-V5-WT plasmid into the pRetroX-Tight-Puro Advanced vector. The confirmed clone is transfected into HEK-293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
L166P the Km-value for methylglyoxal of the mutant enzyme is about 2fold higher compared to the wild-type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
900
-
methylglyoxal 30°C, pH not specified in the publication, wild-type enzyme Homo sapiens
1900
-
methylglyoxal 30°C, pH not specified in the publication, mutant enzyme L166P Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methylglyoxal + H2O Homo sapiens
-
(R)-lactate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q99497 cf. EC 3.5.1.124
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methylglyoxal + H2O
-
Homo sapiens (R)-lactate
-
?

Synonyms

Synonyms Comment Organism
DJ-1
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens