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Literature summary for 4.2.1.125 extracted from
- Molecular cloning, expression, purification, and functional characterization of dammarenediol synthase from Panax ginseng (2013), BioMed Res. Int., 2013, 285740.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, recombinant expression of functional His-tagged enzyme in Escherichia coli strains Origami B (DE3) amd Rosetta (DE3) | Panax ginseng |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 84600 | - |
x * 84600, about, sequence calculation, x * 89000, recombinant His-tagged enzyme, SDS-PAGE | Panax ginseng |
| 89000 | - |
x * 84600, about, sequence calculation, x * 89000, recombinant His-tagged enzyme, SDS-PAGE | Panax ginseng |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3S)-2,3-epoxy-2,3-dihydrosqualene + H2O | Panax ginseng | - |
dammarenediol II | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Panax ginseng | Q08IT1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strains Origami B (DE3) amd Rosetta (DE3) by nickel affinity chromatography | Panax ginseng |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Panax ginseng | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3S)-2,3-epoxy-2,3-dihydrosqualene + H2O | - |
Panax ginseng | dammarenediol II | - |
? | |
| additional information | LC-MS analysis of substrate and product amounts | Panax ginseng | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 84600, about, sequence calculation, x * 89000, recombinant His-tagged enzyme, SDS-PAGE | Panax ginseng |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dammarenediol synthase | - |
Panax ginseng |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is involved in the ginsenoside biosynthetic pathway catalyzing the first committed step, the cyclization of 2,3-oxidosqualene to dammarenediol | Panax ginseng |
| additional information | close correlation of dammarenediol synthetase and saponin production in vitro | Panax ginseng |
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