Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-iminosqualene | effective inhibitor, complete inhibition at 1 mM | Tetrahymena thermophila | |
4,4,10beta-trimethyltransdecal-3beta-ol | 25% inhibition at 40 mM | Tetrahymena thermophila | |
4-chloromercuribenzenesulfonic acid | 86% inhibition at 5 mM | Tetrahymena thermophila | |
diethyldicarbonate | complete inhibition at 5 mM | Tetrahymena thermophila | |
Digitonin | solubilization of the cyclase in 8 mg/ml digitonin inactivates the enzyme, its activity can be recovered by complementation of the assay buffer with octylthioglucoside above its critical micellar concentration | Tetrahymena thermophila | |
Emulgene 911 | solubilization of the cyclase in 1 mg/ml Emulgene 911 inactivates the enzyme | Tetrahymena thermophila | |
additional information | the cyclase is not influenced by EDTA concentrations up to 0.1 M, and lodoacetate is not inhibitory at 1 and 5 mM | Tetrahymena thermophila | |
N,N-Dimethyldodecylamine-N-oxide | effective inhibitor, complete inhibition at 1 mM | Tetrahymena thermophila | |
Ro 43-8522 | effective inhibitor, complete inhibition at 1 mM | Tetrahymena thermophila | |
Triton X-100 | strong inactivator | Tetrahymena thermophila | |
Tween 80 | solubilization of the cyclase in 30 mg/ml Tween 80 inactivates the enzyme, its activity can be recovered by complementation of the assay buffer with octylthioglucoside above its critical micellar concentration | Tetrahymena thermophila |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Tetrahymena thermophila | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
SDS-PAGE | Tetrahymena thermophila |
Organic Solvent | Comment | Organism |
---|---|---|
CHAPS | CHAPS effectively solubilizes the enzyme and maintains its activity | Tetrahymena thermophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tetrahymena thermophila | - |
- |
- |
Tetrahymena thermophila B4-1868 (ATCC 30382) | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-trisacryl column chromatography, hydroxyapatite column chromatography, and Mono Q column chromatography | Tetrahymena thermophila |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
105 | - |
enzyme from homogenate, pH 7.0, 30°C | Tetrahymena thermophila |
31280 | - |
enzyme after 297fold purification, pH 7.0, 30°C | Tetrahymena thermophila |
Storage Stability | Organism |
---|---|
-80°C, whole cells and particulate material, 6 months, no loss of activity | Tetrahymena thermophila |
6°C, octylthioglucoside-solubilized enzyme, one week, almost complete loss of activity | Tetrahymena thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 squalene + 2 H2O | - |
Tetrahymena thermophila | tetrahymanol + diplopterol | diplopterol amounts to less than 2% of the total products formed by enzymatic conversion | ? | |
2 squalene + 2 H2O | - |
Tetrahymena thermophila B4-1868 (ATCC 30382) | tetrahymanol + diplopterol | diplopterol amounts to less than 2% of the total products formed by enzymatic conversion | ? |
Synonyms | Comment | Organism |
---|---|---|
squalene-tetrahymanol cyclase | - |
Tetrahymena thermophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Tetrahymena thermophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the enzyme is not active at 50°C | Tetrahymena thermophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Tetrahymena thermophila |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 8.5 | the enzyme is not active beyond the pH limits of pH 4.5 and 8.0 | Tetrahymena thermophila |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00003 | - |
pH 7.0, 30°C | Tetrahymena thermophila | 2,3-iminosqualene | |
0.00004 | - |
pH 7.0, 30°C | Tetrahymena thermophila | Ro 43-8522 | |
0.00005 | - |
pH 7.0, 30°C | Tetrahymena thermophila | N,N-Dimethyldodecylamine-N-oxide |