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Literature summary for 4.2.1.122 extracted from

  • Hiyama, T.; Sato, T.; Imanaka, T.; Atomi, H.
    The tryptophan synthase beta-subunit paralogs TrpB1 and TrpB2 in Thermococcus kodakarensis are both involved in tryptophan biosynthesis and indole salvage (2014), FEBS J., 281, 3113-3125 .
    View publication on PubMed
Search for more literature for 4.2.1.122

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermococcus kodakarensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0081
-
 indole pH 7.5, 85°C Thermococcus kodakarensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85600
-
 gel filtration Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine + indole Thermococcus kodakarensis the enzyme is involved in L-tryptophan biosynthesis. TrpB2 does not interact with TrpA as in the case of TrpB1. TrpB2 provides an alternate route to generate Trp from serine and free indole (indole salvage) L-tryptophan + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + indole
-
 Thermococcus kodakarensis L-tryptophan + H2O
-
 ?
L-serine + indole the enzyme is involved in L-tryptophan biosynthesis. TrpB2 does not interact with TrpA as in the case of TrpB1. TrpB2 provides an alternate route to generate Trp from serine and free indole (indole salvage) Thermococcus kodakarensis L-tryptophan + H2O
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 49300, calculated from sequence Thermococcus kodakarensis

Synonyms

Synonyms Comment Organism
TrpB2
-
 Thermococcus kodakarensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.39
-
 indole pH 7.5, 85°C Thermococcus kodakarensis

General Information

General Information Comment Organism
malfunction double-deletion mutant (DELTAtrpB1DELTAtrpB2) displays Trp auxotrophy, whereas individual single mutants (DELTAtrpB1 and DELTAtrpB2 strains) do not. To examine the capacity of TrpB1 and TrpB2 in Trp synthesis via indole salvage, DtrpEB1 and DtrpEB2 mutant strains are constructed using strain KUW1 (DELTApyrFDtrpE) as a host, eliminating the route for endogenous indole synthesis. Indole complements the Trp auxotrophies of DELTAtrpEB1 (DELTApyrFDELTAtrpEDELTAtrpB1) and DELTAtrpEB2 (DELTApyrFDELTAtrpEDELTAtrpB2) to similar levels. The results indicate that TrpB1 and TrpB2 both contribute to Trp biosynthesis in Thermococcus kodakarensis and can utilize free indole, and that indolesalvage does not necessarily rely on TrpB2 to a greater extent Thermococcus kodakarensis
metabolism the enzyme is involved in L-tryptophan biosynthesis Thermococcus kodakarensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
48
-
 indole pH 7.5, 85°C Thermococcus kodakarensis