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Literature summary for 4.2.1.12 extracted from

  • Scopes, R.K.; Griffiths-Smith, K.
    Use of differential dye-ligand chromatography with affinity elution for enzyme purification: 6-phosphogluconate dehydratase from Zymomonas mobilis (1984), Anal. Biochem., 136, 530-534.
    View publication on PubMed
Search for more literature for 4.2.1.12

Inhibitors

Inhibitors Comment Organism Structure
glycerophosphates most strongly inhibited by the D-alpha-isomer which structurally corresponds to half of the substrate molecule Zymomonas mobilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 6-phosphogluconate
-
 Zymomonas mobilis

Metals/Ions

Metals/Ions Comment Organism Structure
Iron enzyme contains 3.0 iron atoms per 63000 Da subunit Zymomonas mobilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 2 * 63000, SDS-PAGE Zymomonas mobilis
130000
-
-
 Zymomonas mobilis

Organism

Organism UniProt Comment Textmining
Zymomonas mobilis
-
-
-

Oxidation Stability

Oxidation Stability Organism
rapid inactivation under oxidizing conditions Zymomonas mobilis

Purification (Commentary)

Purification (Comment) Organism
one-step procedure Zymomonas mobilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Zymomonas mobilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate
-
 Zymomonas mobilis 6-phospho-2-dehydro-3-deoxy-D-gluconate + H2O
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 63000, SDS-PAGE Zymomonas mobilis