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Literature summary for 4.2.1.119 extracted from

  • Katano, M.; Takahashi, K.; Hirano, T.; Kazama, Y.; Abe, T.; Tsukaya, H.; Ferjani, A.
    Suppressor screen and phenotype analyses revealed an emerging role of the monofunctional peroxisomal enoyl-CoA hydratase 2 in compensated cell enlargement (2016), Front. Plant Sci., 7, 132 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.119

Cloned(Commentary)

Cloned (Comment) Organism
gene ECH2, DNA and amino acid sequence determination and analysis, map-based cloning Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information mutagenesis of fugu5-1 seeds with heavy-ion irradiation and screening of mutations that restrain compensated cell enlargement (CCE) to gain insight into the genetic pathway(s) involved in CCE. Mutant A#3-1 has a cell size that is severely reduced, but the cell number remains similar to that of original fugu5-1. The cell number decreases in A#3-1 single mutant (A#3-1sm), similar to that of fugu5-1, but cell size is almost equal to that of the wild-type. A#3-1 mutation does not affect CCE in other compensation exhibiting mutant backgrounds, such as an3-4 and fugu2-1/fas1-6. Subsequent map-based cloning combined with genome sequencing and HRM curve analysis identified enoyl-CoA hydratase 2 (ECH2) as the causal gene of A#3-1. The above phenotypes are consistently observed in the ech2-1 allele and supplying sucrose restores the morphological and cellular phenotypes in fugu5-1, ech2-1, A#3-1sm, fugu5-1 ech2-1, and A#3-1; fugu5-1. The ech2-1 mutant allele is indistinguishable from A#3-1sm and suppresses CCEin fugu5. Analysis of ech2-1 mutant. Enoyl-CoA hydratase 2 gene mutation causes the A#3-1sm phenotypes, overview Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
 Arabidopsis thaliana 5777
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E)-2-enoyl-CoA + H2O Arabidopsis thaliana
-
 (3R)-3-hydroxyacyl-CoA
-
 r
(2E)-2-enoyl-CoA + H2O Arabidopsis thaliana Col-0
-
 (3R)-3-hydroxyacyl-CoA
-
 r

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8VYI3 mutant fugu5-1
-
Arabidopsis thaliana Col-0 Q8VYI3 mutant fugu5-1
-

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Arabidopsis thaliana
-
leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E)-2-enoyl-CoA + H2O
-
 Arabidopsis thaliana (3R)-3-hydroxyacyl-CoA
-
 r
(2E)-2-enoyl-CoA + H2O
-
 Arabidopsis thaliana Col-0 (3R)-3-hydroxyacyl-CoA
-
 r

Synonyms

Synonyms Comment Organism
At1g76150
-
 Arabidopsis thaliana
ECH2
-
 Arabidopsis thaliana
peroxisomal enoyl-CoA hydratase 2
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction defects in either HC-PPase or ECH2 compromise cell proliferation due to defects in mobilizing seed storage lipids, phenotype, overview. Enoyl-CoA hydratase 2 (ECH2) gene mutation causes the A#3-1sm phenotypes, overview. Mutant A#3-1 has a cell size that is severely reduced, but the cell number remains similar to that of original fugu5-1. The cell number decreases in A#3-1 single mutant (A#3-1sm), similar to that of fugu5-1, but cell size is almost equal to that of the wild-type. A#3-1 mutation does not affect CCE in other compensation exhibiting mutant backgrounds, such as an3-4 and fugu2-1/fas1-6 Arabidopsis thaliana
physiological function role of the monofunctional peroxisomal enoyl-CoA hydratase 2 in compensated cell enlargement (CCE). Enzyme ECH2 alone likely promotes CCE during the post-mitotic cell expansion stage of cotyledon development, probably by converting indolebutyric acid to indole acetic acid Arabidopsis thaliana