Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant hydratase domains of multifunctional enzyme type 2 hydratase as GFP-tagged protein in Escherichia coli strain JM109 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A348T | site-directed mutagenesis, the mutation does not affect the enzyme | Homo sapiens |
A427V | site-directed mutagenesis, the mutation does not affect the enzyme, the mutant shows a slight increase in activity | Homo sapiens |
A491T | site-directed mutagenesis, the mutation does not affect the enzyme | Homo sapiens |
A606S | site-directed mutagenesis, the mutant shows reduced activity | Homo sapiens |
D510Y | site-directed mutagenesis, inactive mutant, the mutation disrupts active site architecture | Homo sapiens |
E366G | site-directed mutagenesis, inactive mutant, the mutation disrupts dimerization | Homo sapiens |
G533R | site-directed mutagenesis, inactive mutant, the mutation disrupts ligand interaction | Homo sapiens |
H532R | site-directed mutagenesis, inactive mutant, the mutation disrupts active site architecture | Homo sapiens |
I516T | site-directed mutagenesis, the mutation disrupts dimerization, the mutant shows reduced activity | Homo sapiens |
I559V | site-directed mutagenesis, the mutation does not affect the enzyme | Homo sapiens |
L405P | site-directed mutagenesis, inactive mutant, the mutation disrupts ligand interaction | Homo sapiens |
additional information | site-directed mutagenesis is used for construction of mutants corresponding to 17 reported missense mutations of multifunctional enzyme type 2 hydratase. Some mutants are almost or completely inactive causing the D-bifunctional protein deficiency type II | Homo sapiens |
N457D | site-directed mutagenesis, the mutant shows reduced activity | Homo sapiens |
N457Y | site-directed mutagenesis, the mutation disrupts domain folding, the mutant shows reduced activity | Homo sapiens |
P529L | site-directed mutagenesis, inactive mutant, the mutation disrupts active site architecture | Homo sapiens |
R506C | site-directed mutagenesis, inactive mutant, the mutation disrupts dimerization | Homo sapiens |
R506H | site-directed mutagenesis, inactive mutant, the mutation disrupts dimerization | Homo sapiens |
W511R | site-directed mutagenesis, the mutation does not affect the enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Homo sapiens | 5777 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant GFP-tagged hydratase domains of multifunctional enzyme type 2 hydratase from Escherichia coli strain JM109 by anion exchange chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3R)-3-hydroxyhexadecanoyl-CoA | - |
Homo sapiens | (2E)-2-hexadecenoyl-CoA + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MFE2 hydratase | - |
Homo sapiens |
multifunctional enzyme type 2 hydratase | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | inactivating mutations of multifunctional enzyme type 2 hydratase lead to D-bifunctional protein deficiency type II | Homo sapiens |
additional information | MFE2 hydratase is R-specific, while MFE1 hydratase is S-specific | Homo sapiens |