Literature summary for 4.2.1.119 extracted from

Goepfert, S.; Hiltunen, J.K.; Poirier, Y.
Identification and functional characterization of a monofunctional peroxisomal enoyl-CoA hydratase 2 that participates in the degradation of even cis-unsaturated fatty acids in Arabidopsis thaliana (2006), J. Biol. Chem., 281, 35894-35903.

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Cloned(Commentary)

Cloned (Comment)	Organism
AtECH2 contains a peroxisome targeting signal at the C-terminal end, is addressed to the peroxisome in Saccharomyces cerevisiae, and a fusion protein between AtECH2 and a fluorescent protein is targeted to peroxisomes in onion cells. To assess the peroxisomal addressing of AtECH2, a fusion protein between an EYFP at the N terminus and AtECH2 at the C terminus is constructed and expressed under the control of a double cauliflower mosaic virus (CaMV) 35 S viral promoter to allow transient expression of the fusion protein in onion cells following biolistic bombardment. The fluorescence is examined by confocal microscopy after 12 h	Arabidopsis thaliana

Cloned (Comment)

Organism

AtECH2 contains a peroxisome targeting signal at the C-terminal end, is addressed to the peroxisome in Saccharomyces cerevisiae, and a fusion protein between AtECH2 and a fluorescent protein is targeted to peroxisomes in onion cells. To assess the peroxisomal addressing of AtECH2, a fusion protein between an EYFP at the N terminus and AtECH2 at the C terminus is constructed and expressed under the control of a double cauliflower mosaic virus (CaMV) 35 S viral promoter to allow transient expression of the fusion protein in onion cells following biolistic bombardment. The fluorescence is examined by confocal microscopy after 12 h

Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
peroxisome	-	Arabidopsis thaliana	5777	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
34000	-	x * 34000, calculated from sequence	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(3R)-3-hydroxyacyl-CoA	Arabidopsis thaliana	AtECH2 participates in vivo in the conversion of the intermediate (3R)-hydroxyacyl-CoA, generated by the metabolism of fatty acids with a cis (Z)-unsaturated bond on an even-numbered carbon, to the (2E)-enoyl-CoA for further degradation through the core beta-oxidation cycle. AtECH2 is a monofunctional enzyme in Arabidopsis thaliana that is devoid of 3-hydroxyacyl-CoA dehydrogenase activity	(2E)-2-enoyl-CoA + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q8VYI3	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
flower bud	-	Arabidopsis thaliana	-
leaf	AtECH2 gene expression is strongest in tissues with high beta-oxidation activity, such as germinating seedlings and senescing leaves	Arabidopsis thaliana	-
root	-	Arabidopsis thaliana	-
seedling	AtECH2 gene expression is strongest in tissues with high beta-oxidation activity, such as germinating seedlings and senescing leaves	Arabidopsis thaliana	-
stem	-	Arabidopsis thaliana	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.2	-	0.03 mM (2E)-hexadecenoyl-CoA as a substrate	Arabidopsis thaliana
3.5	-	0.03 m (2E)-hexenoyl-CoA as a substrate	Arabidopsis thaliana
12.4	-	0.1 mM, 3-hydroxydecanoyl-CoA as a substrate	Arabidopsis thaliana
30	-	0.05 mM (2E)-decenoyl-CoA as a substrate	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
(2E)-decenoyl-CoA + H2O	-	Arabidopsis thaliana	(3R)-3-hydroxydecanoyl-CoA	-	r
(2E)-hexadecenoyl-CoA + H2O	-	Arabidopsis thaliana	(3R)-3-hydroxyhexadecanoyl-CoA	-	?
(2E)-hexenoyl-CoA + H2O	-	Arabidopsis thaliana	(3R)-3-hydroxyhexanoyl-CoA	-	?
(3R)-3-hydroxyacyl-CoA	AtECH2 participates in vivo in the conversion of the intermediate (3R)-hydroxyacyl-CoA, generated by the metabolism of fatty acids with a cis (Z)-unsaturated bond on an even-numbered carbon, to the (2E)-enoyl-CoA for further degradation through the core beta-oxidation cycle. AtECH2 is a monofunctional enzyme in Arabidopsis thaliana that is devoid of 3-hydroxyacyl-CoA dehydrogenase activity	Arabidopsis thaliana	(2E)-2-enoyl-CoA + H2O	-	?

Subunits

Subunits	Comment	Organism
?	x * 34000, calculated from sequence	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
AtECH2	gene name. Alignment of AtECH2 with homologous proteins is shown	Arabidopsis thaliana
AtECH2	monofunctional enzyme in Arabidopsis thaliana	Arabidopsis thaliana
enoyl-CoA hydratase 2	-	Arabidopsis thaliana
enoyl-CoA hydratase 2	monofunctional enzyme in Arabidopsis thaliana	Arabidopsis thaliana

pI Value

Organism	Comment	pI Value Maximum	pI Value
Arabidopsis thaliana	calculated from sequence	-	7.6