Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Candida tropicalis |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour-diffusion method. Crystals of native and SeMet CtMfe2p(dha+bdelta) | Candida tropicalis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | CtMfe2p(dha+bdelta) labelled with selenomethionine (SeMet), the plasmid pET3a::CtMfe2p(dha+bdelta) is transformed to the methionine-auxotrophic Escherichia coli strain B834(DE3). The incorporation of SeMet into the structure does not affect the hydratase 2 activity | Candida tropicalis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
both recombinant CtMfe2p(dha+bdelta) and its SeMet analogue, SDS-PAGE | Candida tropicalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Candida tropicalis | in yeast, the second and the third reaction of the fatty-acid beta-oxidation spiral are catalysed by peroxisomal multifunctional enzyme type 2 (Mfe2p/Fox2p). This protein has two (3R)-hydroxyacyl-CoA dehydrogenase domains and a C-terminal 2-enoyl-CoA hydratase 2 domain | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida tropicalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Candida tropicalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E)-2-decenoyl-CoA + H2O | activity measurements are based on the formation of the magnesium complex of 3-ketoacyl-CoA from (2E)-2-decenoyl-CoA | Candida tropicalis | (3R)-3-hydroxydecanoyl-CoA | - |
? | |
additional information | in yeast, the second and the third reaction of the fatty-acid beta-oxidation spiral are catalysed by peroxisomal multifunctional enzyme type 2 (Mfe2p/Fox2p). This protein has two (3R)-hydroxyacyl-CoA dehydrogenase domains and a C-terminal 2-enoyl-CoA hydratase 2 domain | Candida tropicalis | ? | - |
? | |
additional information | MFE-2 is a multifunctional enzyme with 2-enoyl-CoA hydratase 2 activity and 2/(3R)-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.36) activity | Candida tropicalis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 63000, both recombinant CtMfe2p(dha+bdelta) and its SeMet analogue, SDS-PAGE | Candida tropicalis |
Synonyms | Comment | Organism |
---|---|---|
2-enoyl-CoA hydratase 2 | - |
Candida tropicalis |
MFE-2 | multifunctional enzyme | Candida tropicalis |
MFE-2 | multifunctional enzyme with 2-enoyl-CoA hydratase 2 activity and 2/(3R)-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.36) activity | Candida tropicalis |
Mfe2p [CtMfe2p(dha+bdelta)] | 2-enoyl-CoA hydratase 2 domain of Candida tropicalis | Candida tropicalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
388 | - |
(2E)-2-decenoyl-CoA | recombinant enzyme CtMfe2p(dha+bdelta) | Candida tropicalis |